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DOC2A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS646341

There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which… More details

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Product Overview

Online Datasheet in PDF
Product Name
DOC2A, Polyclonal Antibody
Full Product Name
DOC2A, ID (DOC2A, Double C2-like domain-containing protein alpha)
Product Gene Name
anti-DOC2A antibody
Product Synonym Names
Anti -DOC2A, ID (DOC2A, Double C2-like domain-containing protein alpha)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
43,959 Da
Chromosome Location
Chromosome: 16; NC_000016.9 (30016835..30023424, complement). Location: 16p11.2
OMIM
604567
3D Structure
ModBase 3D Structure for Q14183
Immunogen
DOC2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 284-314 amino acids from the Central region of human DOC2A.

Product Categories/Family for anti-DOC2A antibody

Antibodies; Abs to Proteins
Applications Tested/Suitable for anti-DOC2A antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-DOC2A antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50

Flow Cytometry (FC/FACS) of anti-DOC2A antibody

DOC2A Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-DOC2A Polyclonal Antibody – Cat# MBS646341

Western Blot (WB) of anti-DOC2A antibody

DOC2A Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DOC2A antibody detected the DOC2A protein (arrow).
Rabbit Anti-DOC2A Polyclonal Antibody, Western Blot (WB) – Cat# MBS646341

Immunohistochemistry (IHC) of anti-DOC2A antibody

DOC2A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DOC2A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-DOC2A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS646341

Other Notes

Small volumes of anti-DOC2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DOC2A purchase

MBS646341 is an antibody targeting DOC2A. Also known as DOC2A, ID (DOC2A, Double C2-like domain-containing protein alpha). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DOC2A. It may not necessarily be applicable to this product.

NCBI GI #
530719517
NCBI GeneID
8448
NCBI Accession #
NP_001268997.1
UniProt Primary Accession #
Q14183
UniProt Secondary Accession #
Q6P4G4; Q7Z5G0; Q8IVX0
UniProt Related Accession #
Q14183
GenBank Nucleotide #
NM_001282068.1
NCBI Official Full Name
double C2-like domain-containing protein alpha
NCBI Official Synonym Full Names
double C2-like domains, alpha
NCBI Official Symbol
DOC2A
NCBI Official Synonym Symbols
Doc2
UniProt Protein Name
Double C2-like domain-containing protein alpha
Protein Family
Double C2-like domain-containing protein
UniProt Gene Name
DOC2A
UniProt Synonym Gene Names
Doc2; Doc2-alpha
UniProt Entry Name
DOC2A_HUMAN
NCBI Protein Information
double C2-like domain-containing protein alpha; doc2-alpha

NCBI Summary for DOC2A

There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2A is mainly expressed in brain and is suggested to be involved in Ca(2+)-dependent neurotransmitter release. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
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UniProt Comments for DOC2A

Function: Calcium sensor which most probably regulates fusion of vesicles with membranes. Binds calcium and phospholipids. May be involved in calcium dependent neurotransmitter release through the interaction with UNC13A. May be involved in calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Regulates Ca2+-dependent secretory lysosome exocytosis in mast cells. Ref.4 Ref.5 Ref.6

Subunit structure: Interacts (via N-terminus) with UNC13A. Interacts with cytoplasmic dynein light chain DYNLT1. Interacts with UNC13D. Ref.4 Ref.5 Ref.6

Subcellular location: Lysosome

By similarity. Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Peripheral membrane protein

Probable. Cell junction › synapse › synaptosome. Note: Colocalizes to synaptic vesicles.

Tissue specificity: Predominantly expressed in brain. Also expressed in testis. Ref.1 Ref.3

Domain: C2 domain 1 is involved in binding calcium and phospholipids

By similarity.

Sequence similarities: Contains 2 C2 domains.
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Research Articles on DOC2A

1. Study analyzed Doc2alpha and Doc2beta and found that Doc2 responds to changes in [Ca2+], with markedly slower kinetics as compared to the cytosolic domain of syt I (syt), and operates on a timescale consistent with asynchronous neurotransmitter release.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DOC2A antibody

 Reference Product  PubMed Publications
 UNC13B antibody  >6 publications with DOC2A and UNC13B
 SEZ6L2 antibody  >1 publications with DOC2A and SEZ6L2
 DYNLT1 antibody  >1 publications with DOC2A and DYNLT1

Diseases associated with anti-DOC2A antibody

 Disease Name  Pubmed Publications
 Arthritis Antibodies  >2 publications with DOC2A and Arthritis
 Autoimmune Diseases Antibodies  >1 publications with DOC2A and Autoimmune Diseases
 Bone Diseases Antibodies  >1 publications with DOC2A and Bone Diseases
 Brain Diseases Antibodies  >1 publications with DOC2A and Brain Diseases
 Nervous System Diseases Antibodies  >1 publications with DOC2A and Nervous System Diseases

Organs/Tissues associated with anti-DOC2A antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >16 publications with DOC2A and Brain
 Bone Antibodies  >2 publications with DOC2A and Bone
 Embryonic Tissue Antibodies  >1 publications with DOC2A and Embryonic Tissue
 Lung Antibodies  >1 publications with DOC2A and Lung
 Blood Antibodies  >1 publications with DOC2A and Blood
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