CYP2B6 Antibody – Rabbit Polyclonal

Function: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,4-cineole 2-exo-monooxygenase. Ref.7 Ref.8 Ref.9 Ref.10

Catalytic activity: 1,4-cineole + NADPH + O2 = 2-exo-hydroxy-1,4-cineole + NADP+ + H2O. Ref.7

Cofactor: Heme group. Ref.8 Ref.9 Ref.10

Subcellular location: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity: Expressed in liver, lung and heart right ventricle. Ref.6

Induction: By phenobarbital.

Post-translational modification: Phosphorylation is accompanied by a decrease in enzyme activity

By similarity.

Polymorphism: Genetic variations in CYP2B6 are responsible for poor metabolism of efavirenz and, therefore, susceptibility to efavirenz toxicity in the central nervous system [

MIM:614546]. Efavirenz is a non-nucleoside reverse transcriptase inhibitor frequently prescribed with 2 nucleoside reverse transcriptase inhibitors as initial therapy for human immunodeficiency virus (HIV) infection. Up to half of patients treated with efavirenz, experience side effects in the central nervous system, including dizziness, insomnia, impaired concentration, somnolence, and abnormal dreams. Severe depression, aggressive behavior, and paranoid or manic reactions may also occur, depending on efavirenz concentration in the plasma.

Sequence similarities: Belongs to the cytochrome P450 family.

Biophysicochemical propertiesKinetic parameters:KM=360 µM for 1,4-cineole Ref.7Vmax=3.4 nmol/min/nmol enzyme toward 2-exo-hydroxy-1,4-cineole