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ARID3A Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS646424

Transcription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation. More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ARID3A, Monoclonal Antibody
Full Product Name
ARID3A (AT-rich Interactive Domain-containing Protein 3A, ARID Domain-containing Protein 3A, B-cell Regulator of IgH Transcription, Bright, Dead Ringer-like Protein 1, E2F-binding Protein 1, DRIL1, DRIL3, DRX, E2FBP1)
Product Gene Name
anti-ARID3A antibody
Product Synonym Names
Anti -ARID3A (AT-rich Interactive Domain-containing Protein 3A, ARID Domain-containing Protein 3A, B-cell Regulator of IgH Transcription, Bright, Dead Ringer-like Protein 1, E2F-binding Protein 1, DRIL1, DRIL3, DRX, E2FBP1)
Immunogen Sequence
QYMKYLYPYE CEKRGLSNPN ELQAAIDSNR REGRRQSFGG SLFAYSPGGA HGMLSSPKLP VSSLGLAAST NGSSITPAPK IKKEEDSAIP ITVPGRLPVS
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1A11
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human ARID3A.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
62,889 Da
Chromosome Location
Chromosome: 19; NC_000019.9 (925733..972816). Location: 19p13.3
OMIM
603265
3D Structure
ModBase 3D Structure for Q99856
Immunogen
Partial recombinant corresponding to aa317-416 from human ARID3A (NP_005215) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-ARID3A antibody

Antibodies; Abs to Binding Proteins
Applications Tested/Suitable for anti-ARID3A antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-ARID3A antibody
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Dilution: Immunofluorescence: 10ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml

Western Blot (WB) of anti-ARID3A antibody

Western Blot detection against Immunogen (36.74kD).
Mouse Anti-ARID3A Monoclonal Antibody – Cat# MBS646424

Western Blot (WB) of anti-ARID3A antibody

ARID3A monoclonal antibody, Western Blot analysis of ARID3A expression in K-562.
Mouse Anti-ARID3A Monoclonal Antibody, Western Blot (WB) – Cat# MBS646424

Immunohistochemistry (IHC) of anti-ARID3A antibody

Immunoperoxidase of monoclonal antibody to ARID3A on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].
Mouse Anti-ARID3A Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS646424

Immunofluorescence (IF) of anti-ARID3A antibody

Immunofluorescence of monoclonal antibody to ARID3A on HeLa cell. [antibody concentration 10ug/ml].
Mouse Anti-ARID3A Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS646424

Testing Data of anti-ARID3A antibody

Detection limit for recombinant GST tagged ARID3A is ~0.03ng/ml as a capture antibody.
Mouse Anti-ARID3A Monoclonal Antibody, Testing Data – Cat# MBS646424

Western Blot (WB) of anti-ARID3A antibody

Western blot analysis of ARID3A over-expressed 293 cell line, cotransfected with ARID3A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ARID3A monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-ARID3A Monoclonal Antibody, Western Blot (WB) – Cat# MBS646424

Other Notes

Small volumes of anti-ARID3A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ARID3A purchase

MBS646424 is an antibody targeting ARID3A. Also known as ARID3A (AT-rich Interactive Domain-containing Protein 3A, ARID Domain-containing Protein 3A, B-cell Regulator of IgH Transcription, Bright, Dead Ringer-like Protein 1, E2F-binding Protein 1, DRIL1, DRIL3, DRX, E2FBP1). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ARID3A. It may not necessarily be applicable to this product.

NCBI GI #
4885193
NCBI GeneID
1820
NCBI Accession #
NP_005215.1
UniProt Primary Accession #
Q99856
UniProt Secondary Accession #
Q5I858; Q6P9C6; Q8IZA7; Q8N4Z3
UniProt Related Accession #
Q99856
GenBank Nucleotide #
NM_005224.2
NCBI Official Full Name
AT-rich interactive domain-containing protein 3A
NCBI Official Synonym Full Names
AT rich interactive domain 3A (BRIGHT-like)
NCBI Official Symbol
ARID3A
NCBI Official Synonym Symbols
DRIL1; DRIL3; BRIGHT; E2FBP1
UniProt Protein Name
AT-rich interactive domain-containing protein 3A
UniProt Synonym Protein Names
B-cell regulator of IgH transcription; Bright; Dead ringer-like protein 1; E2F-binding protein 1
Protein Family
AT-rich interactive domain-containing protein
UniProt Gene Name
ARID3A
UniProt Synonym Gene Names
DRIL1; DRIL3; DRX; E2FBP1; ARID domain-containing protein 3A; Bright
UniProt Entry Name
ARI3A_HUMAN
NCBI Protein Information
AT-rich interactive domain-containing protein 3A; dead ringer-like 1; E2F-binding protein 1; ARID domain-containing 3A; dead ringer-like protein 1; ARID domain-containing protein 3A; B-cell regulator of IgH transcription; AT rich interactive domain 3A (BRIGHT- like) protein

NCBI Summary for ARID3A

This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA binding proteins. It was found by homology to the Drosophila dead ringer gene, which is important for normal embryogenesis. Other ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation, and possibly in chromatin structure modification. [provided by RefSeq, Jul 2008]
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UniProt Comments for ARID3A

Function: Transcription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation. Ref.6 Ref.7

Subunit structure: Homodimer. Heterodimer with ARID3B. Interacts with E2F1. Interacts with GTF2I and BTK. Ref.2 Ref.9 Ref.10

Subcellular location: Nucleus. Cytoplasm. Note: Shuttles between nucleus and cytoplasm. Ref.10

Tissue specificity: Widely expressed, with highest expression in skeletal muscle, thalamus, and colon.

Induction: By p53/TP53 following DNA damage. Ref.7

Sequence similarities: Contains 1 ARID domain.Contains 1 REKLES domain.

Sequence caution: The sequence AAW30734.1 differs from that shown. Reason: Frameshift at position 498.
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Research Articles on ARID3A

1. miR-125b can act as an oncogene in B-cell acute lymphoblastic leukemia by targeting ARID3a and mediating its repression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ARID3A antibody

 Reference Product  PubMed Publications
 E2F1 antibody  >7 publications with ARID3A and E2F1
 BTK antibody  >6 publications with ARID3A and BTK
 PML antibody  >4 publications with ARID3A and PML
 SP140 antibody  >1 publications with ARID3A and SP140
 TP53 antibody  >1 publications with ARID3A and TP53

Pathways associated with anti-ARID3A antibody

 Products by Pathway  Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram

Diseases associated with anti-ARID3A antibody

 Disease Name  Pubmed Publications
 Translocation, Genetic Antibodies  >1 publications with ARID3A and Translocation, Genetic
 Brain Diseases Antibodies  >1 publications with ARID3A and Brain Diseases
 Kidney Diseases Antibodies  >1 publications with ARID3A and Kidney Diseases

Organs/Tissues associated with anti-ARID3A antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >3 publications with ARID3A and Bone
 Ovary Antibodies  >1 publications with ARID3A and Ovary
 Liver Antibodies  >1 publications with ARID3A and Liver
 Kidney Antibodies  >1 publications with ARID3A and Kidney
 Lymph Node Antibodies  >1 publications with ARID3A and Lymph Node
 Embryonic Tissue Antibodies  >1 publications with ARID3A and Embryonic Tissue
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