Rabbit Anti-DCAF12 Polyclonal Antibody – Cat# MBS646491

WDR40A Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the WDR40A antibody detected the WDR40A protein (arrow).

DCAF12 Antibody – Rabbit Polyclonal

Name: DCAF12 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS646491
Price: 895 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS646491

This gene encodes a WD repeat-containing protein that interacts with the COP9 signalosome, a macromolecular complex that interacts with cullin-RING E3 ligases and regulates their activity by hydrolyzing cullin-Nedd8 conjugates. More details

$895 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

WDR40A (DCAF12), Polyclonal Antibody

Full Product Name

WDR40A, ID (DCAF12, KIAA1892, TCC52, WDR40A, DDB1- and CUL4-associated factor 12, Centrosome-related protein TCC52, Testis cancer centrosome-related protein, WD repeat-containing protein 40A)

Product Gene Name

anti-DCAF12 antibody

Product Synonym Names

Anti -WDR40A, ID (DCAF12, KIAA1892, TCC52, WDR40A, DDB1- and CUL4-associated factor 12, Centrosome-related protein TCC52, Testis cancer centrosome-related protein, WD repeat-containing protein 40A)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Human

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

50,517 Da

Chromosome Location

Chromosome: 9; NC_000009.11 (34086381..34126771, complement). Location: 9p13.3

3D Structure

ModBase 3D Structure for Q5T6F0

Immunogen

WDR40A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 222-252 amino acids from the Central region of human WDR40A.

Product Categories/Family for anti-DCAF12 antibody

Antibodies; Abs to Cancer Markers

Applications Tested/Suitable for anti-DCAF12 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-DCAF12 antibody

Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Other Notes

Small volumes of anti-DCAF12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DCAF12 purchase

MBS646491 is an antibody targeting DCAF12. Also known as WDR40A, ID (DCAF12, KIAA1892, TCC52, WDR40A, DDB1- and CUL4-associated factor 12, Centrosome-related protein TCC52, Testis cancer centrosome-related protein, WD repeat-containing protein 40A). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DCAF12. It may not necessarily be applicable to this product.

NCBI GI #

34782819

NCBI GeneID

25853

NCBI Accession #

AAH08893.2

UniProt Primary Accession #

Q5T6F0

UniProt Secondary Accession #

Q5T6E9; Q5T6F1; Q6P3V0; Q7L4F8; Q96PZ5; Q9NXA9; Q9UFJ1; A8KA70; D3DRL6

UniProt Related Accession #

Q5T6F0

NCBI Official Full Name

WDR40A protein, partial

NCBI Official Synonym Full Names

DDB1 and CUL4 associated factor 12

NCBI Official Symbol

DCAF12

NCBI Official Synonym Symbols

CT102; TCC52; WDR40A; KIAA1892

UniProt Protein Name

DDB1- and CUL4-associated factor 12

UniProt Synonym Protein Names

Centrosome-related protein TCC52; Testis cancer centrosome-related protein; WD repeat-containing protein 40A

Protein Family

DDB1- and CUL4-associated factor

UniProt Gene Name

DCAF12

UniProt Synonym Gene Names

KIAA1892; TCC52; WDR40A

UniProt Entry Name

DCA12_HUMAN

NCBI Protein Information

DDB1- and CUL4-associated factor 12; WD repeat domain 40A; cancer/testis antigen 102; WD repeat-containing protein 40A; centrosome-related protein TCC52; testis cancer centrosome-related protein

UniProt Comments for DCAF12

Function: May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. Ref.8

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Interacts with DDB1. Ref.8

Subcellular location: Cytoplasm. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome Ref.9.

Tissue specificity: Highly expressed in lung cancer tissues and some cancer cell lines. Restricted expression in normal testis. Ref.9

Sequence similarities: Belongs to the WD repeat DCAF12 family.Contains 4 WD repeats.

Sequence caution: The sequence BAA91106.1 differs from that shown. Reason: Frameshift at position 27. The sequence BAB67785.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI15300.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI15302.1 differs from that shown. Reason: Erroneous gene model prediction.

Research Articles on DCAF12

1. Of the classifier's 11 informative genes, expression of MIR and WDR40 showed statistically significant increases for both Grade 1B and Grade >or=3A rejection.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.