Mouse Anti-CEP250 Monoclonal Antibody – Cat# MBS646554

Western Blot detection against Immunogen (37kD).

CEP250 Antibody – Mouse Monoclonal

Name: CEP250 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS646554
Price: 880 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS646554

MBS646554 is an antibody targeting Cep250. Also known as CEP250 (Centrosome-associated Protein CEP250, 250 kDa Centrosomal Protein, Cep250, Centrosomal Nek2-associated Protein 1, C-Nap1, Centrosomal Protein 2, CEP2, CNAP1). Reactive in Human. Host: Mouse.… More details

$880 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CEP250, Monoclonal Antibody

Full Product Name

CEP250 (Centrosome-associated Protein CEP250, 250 kDa Centrosomal Protein, Cep250, Centrosomal Nek2-associated Protein 1, C-Nap1, Centrosomal Protein 2, CEP2, CNAP1)

Product Gene Name

anti-Cep250 antibody

Product Synonym Names

Anti -CEP250 (Centrosome-associated Protein CEP250, 250 kDa Centrosomal Protein, Cep250, Centrosomal Nek2-associated Protein 1, C-Nap1, Centrosomal Protein 2, CEP2, CNAP1)

Clonality

Monoclonal

Isotype

IgG2a,k

Clone Number

4A1

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Recognizes human CEP250.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

276,814 Da

Chromosome Location

Chromosome: 2; NC_000068.7 (155956558..155998900). Location: 2 H1; 2

3D Structure

ModBase 3D Structure for Q60952

Immunogen

Partial recombinant corresponding to aa141-240 from CEP250 (AAH01433) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-Cep250 antibody

Antibodies; Abs to Centrosomal Proteins

Applications Tested/Suitable for anti-Cep250 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-Cep250 antibody

Suitable for use in ELISA and Western Blot.

Other Notes

Small volumes of anti-Cep250 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Cep250. It may not necessarily be applicable to this product.

NCBI GI #

194097345

NCBI GeneID

16328

NCBI Accession #

NP_001123471.1

UniProt Primary Accession #

Q60952

UniProt Secondary Accession #

Q2I8G3; Q3UTR4; Q6PFF6; Q8BLC6; E9QMB2

UniProt Related Accession #

Q60952

GenBank Nucleotide #

NM_001129999.1

NCBI Official Full Name

centrosome-associated protein CEP250 isoform 2

NCBI Official Synonym Full Names

centrosomal protein 250

NCBI Official Symbol

Cep250

NCBI Official Synonym Symbols

Cep2; Inmp; AW490617; B230210E21Rik

UniProt Protein Name

Centrosome-associated protein CEP250

UniProt Synonym Protein Names

250 kDa centrosomal protein; Cep250; Centrosomal Nek2-associated protein 1; C-Nap1; Centrosomal protein 2; Intranuclear matrix protein

Protein Family

Centrosome-associated protein

UniProt Gene Name

Cep250

UniProt Synonym Gene Names

Cep2; Inmp; Cep250; C-Nap1

UniProt Entry Name

CP250_MOUSE

NCBI Protein Information

centrosome-associated protein CEP250; C-Nap1; 250 kDa centrosomal protein; intranuclear matrix protein; centrosomal Nek2-associated protein 1

UniProt Comments for Cep250

Function: Probably plays an important role in centrosome cohesion during interphase

By similarity.

Subunit structure: Monomer and homodimer

Probable. Forms a complex in vitro with both NEK2 kinase and the PPP1CC catalytic subunit of protein phosphatase 1 (PP1). Interacts with CEP135

By similarity. Interacts with CROCC/rootletin. Ref.1

Subcellular location: Cytoplasm › perinuclear region. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome › centriole. Cytoplasm › cytoskeleton › cilium basal body. Note: Component of the core centrosome where it is found at the proximal ends of centrioles. In photoreceptors, found at the proximal ends of basal bodies. Ref.1

Post-translational modification: C-terminal part is phosphorylated by NEK2. Dephosphorylated in vitro by the PP1 phosphatase

By similarity.

Sequence caution: The sequence AAB41824.1 differs from that shown. Reason: Frameshift at positions 1843, 1914, 1924, 2341 and 2371.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Cep250 antibody

Products by Pathway	Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Centrosome Maturation Pathway antibodies	Centrosome Maturation Pathway Diagram
G2/M Transition Pathway antibodies	G2/M Transition Pathway Diagram
Loss Of Nlp From Mitotic Centrosomes Pathway antibodies	Loss Of Nlp From Mitotic Centrosomes Pathway Diagram
Loss Of Proteins Required For Interphase Microtubule OrganizationÃ‚Â From The Centrosome Pathway antibodies	Loss Of Proteins Required For Interphase Microtubule OrganizationÃ‚Â From The Centrosome Pathway Diagram
Mitotic G2-G2/M Phases Pathway antibodies	Mitotic G2-G2/M Phases Pathway Diagram
Recruitment Of Mitotic Centrosome Proteins And Complexes Pathway antibodies	Recruitment Of Mitotic Centrosome Proteins And Complexes Pathway Diagram

Organs/Tissues associated with anti-Cep250 antibody

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>1 publications with Cep250 and Testis