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PEPCK2 (PCK2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS646670

This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene… More details

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Product Overview

Online Datasheet in PDF
Product Name
PEPCK2, Polyclonal Antibody
Full Product Name
PEPCK2 (Phosphoenolpyruvate Carboxykinase [GTP], Mitochondrial, PEPCK-M, Phosphoenolpyruvate Carboxylase, PCK2)
Product Gene Name
anti-PCK2 antibody
Product Synonym Names
Anti -PEPCK2 (Phosphoenolpyruvate Carboxykinase [GTP], Mitochondrial, PEPCK-M, Phosphoenolpyruvate Carboxylase, PCK2)
Immunogen Sequence
MAALYRPGLR LNWHGLSPLG WPSCRSIQTL RVLSGDLGQL PTGIRDFVEH SARLCQPEGI HICDGTEAEN TATLTLLEQQ GLIRKLPKYN NCWLARTDPK DVARVESKTV IVTPSQRDTV PLPPGGARGQ LGNWMSPADF QRAVDERFPG CMQGRTMYVL PFSMGPVGSP LSRIGVQLTD SAYVVASMRI MTRLGTPVLQ ALGDGDFVKC LHSVGQPLTG QGEPVSQWPC NPEKTLIGHV PDQREIISFG SGYGGNSLLG KKCFALRIAS RLARDEGWLA EHMLILGITS PAGKKRYVAA AFPSACGKTN LAMMRPALPG WKVECVGDDI AWMRFDSEGR LRAINPENGF FGVAPGTSAT TNPNAMATIQ SNTIFTNVAE TSDGGVYWEG IDQPLPPGVT VTSWLGKPWK PGDKEPCAHP NSRFCAPARQ CPIMDPAWEA PEGVPIDAII FGGRRPKGVP LVYEAFNWRH GVFVGSAMRS ESTAAAEHKG KIIMHDPFAM RPFFGYNFGH YLEHWLSMEG RKGAQLPRIF HVNWFRRDEA GHFLWPGFGE NARVLDWICR RLEGEDSARE TPIGLVPKEG ALDLSGLRAI DTTQLFSLPK DFWEQEVRDI RSYLTEQVNQ DLPKEVLAEL EALERRVHKM
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human PCK2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
70,730 Da
Chromosome Location
Chromosome: 14; NC_000014.8 (24563347..24573339). Location: 14q11.2
OMIM
261650
3D Structure
ModBase 3D Structure for Q16822
Immunogen
Full length human PCK2, aa1-640 (AAH01454.1).

Product Categories/Family for anti-PCK2 antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-PCK2 antibody
Western Blot (WB)
Application Notes for anti-PCK2 antibody
Suitable for use in Western Blot.

Western Blot (WB) of anti-PCK2 antibody

PCK2 rabbit polyclonal antibody. Western Blot analysis of PCK2 expression in human liver.
Rabbit Anti-PCK2 Polyclonal Antibody – Cat# MBS646670

Western Blot (WB) of anti-PCK2 antibody

PCK2 rabbit polyclonal antibody. Western Blot analysis of PCK2 expression in HepG2.
Rabbit Anti-PCK2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS646670

Western Blot (WB) of anti-PCK2 antibody

Western Blot analysis of PCK2 expression in transfected 293T cell line by PCK2 polyclonal antibody. Lane 1: PCK2 transfected lysate (70.7kD). Lane 2: Non-transfected lysate.
Rabbit Anti-PCK2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS646670

Other Notes

Small volumes of anti-PCK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PCK2 purchase

MBS646670 is an antibody targeting PCK2. Also known as PEPCK2 (Phosphoenolpyruvate Carboxykinase [GTP], Mitochondrial, PEPCK-M, Phosphoenolpyruvate Carboxylase, PCK2). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PCK2. It may not necessarily be applicable to this product.

NCBI GI #
66346723
NCBI GeneID
5106
NCBI Accession #
NP_001018083.1
UniProt Primary Accession #
Q16822
UniProt Secondary Accession #
O43253; Q86U01; Q9BV62
UniProt Related Accession #
Q16822
GenBank Nucleotide #
NM_001018073.1
NCBI Official Full Name
phosphoenolpyruvate carboxykinase
NCBI Official Synonym Full Names
phosphoenolpyruvate carboxykinase 2 (mitochondrial)
NCBI Official Symbol
PCK2
NCBI Official Synonym Symbols
PEPCK; PEPCK2; PEPCK-M
UniProt Protein Name
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
UniProt Synonym Protein Names
Phosphoenolpyruvate carboxylase
Protein Family
Phosphoenolpyruvate carboxykinase
UniProt Gene Name
PCK2
UniProt Synonym Gene Names
PEPCK2; PEPCK-M
UniProt Entry Name
PCKGM_HUMAN
NCBI Protein Information
phosphoenolpyruvate carboxykinase [GTP], mitochondrial; phosphoenolpyruvate carboxykinase [GTP], mitochondrial; PEP carboxykinase; phosphopyruvate carboxylase; phosphoenolpyruvate carboxylase

UniProt Comments for PCK2

Function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle

By similarity.

Catalytic activity: GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

Cofactor: Manganese

By similarity.Binds 1 manganese ion per subunit

By similarity.

Pathway: Carbohydrate biosynthesis; gluconeogenesis.

Subunit structure: Monomer.

Subcellular location: Mitochondrion.

Involvement in disease: Mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD) [MIM:261650]: Metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autopsy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.Note: The disease is caused by mutations affecting the gene represented in this entry.

Miscellaneous: In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

Sequence similarities: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Sequence caution: The sequence CAD62600.1 differs from that shown. Reason: Erroneous initiation.
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Research Articles on PCK2

1. expression of HCV nonstructural component NS5A in Huh7 or primary hepatocytes stimulated PEPCK gene expression and glucose output in HepG2 cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PCK2 antibody

 Reference Product  PubMed Publications
 INS antibody  >1 publications with PCK2 and INS
 AKT1 antibody  >1 publications with PCK2 and AKT1

Pathways associated with anti-PCK2 antibody

 Products by Pathway  Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Citrate Cycle (TCA Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle) Pathway Diagram
 Citrate Cycle (TCA Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle) Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram

Diseases associated with anti-PCK2 antibody

 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >5 publications with PCK2 and Fatty Liver
 Carcinoma, Hepatocellular Antibodies  >3 publications with PCK2 and Carcinoma, Hepatocellular
 Neoplasms Antibodies  >3 publications with PCK2 and Neoplasms
 Liver Diseases Antibodies  >2 publications with PCK2 and Liver Diseases
 Liver Neoplasms Antibodies  >2 publications with PCK2 and Liver Neoplasms
 Adenocarcinoma Antibodies  >2 publications with PCK2 and Adenocarcinoma
 Disease Models, Animal Antibodies  >1 publications with PCK2 and Disease Models, Animal
 Weight Loss Antibodies  >1 publications with PCK2 and Weight Loss
 Hepatitis Antibodies  >1 publications with PCK2 and Hepatitis
 Inflammation Antibodies  >1 publications with PCK2 and Inflammation

Organs/Tissues associated with anti-PCK2 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >13 publications with PCK2 and Liver
 Blood Antibodies  >10 publications with PCK2 and Blood
 Adipose Tissue Antibodies  >9 publications with PCK2 and Adipose Tissue
 Connective Tissue Antibodies  >5 publications with PCK2 and Connective Tissue
 Kidney Antibodies  >4 publications with PCK2 and Kidney
 Embryonic Tissue Antibodies  >3 publications with PCK2 and Embryonic Tissue
 Pancreas Antibodies  >3 publications with PCK2 and Pancreas
 Muscle Antibodies  >2 publications with PCK2 and Muscle
 Heart Antibodies  >2 publications with PCK2 and Heart
 Placenta Antibodies  >1 publications with PCK2 and Placenta
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