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YI016 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS646784

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (By similarity). More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
YI016, Polyclonal Antibody
Full Product Name
YI016, CT (Putative tubulin beta chain-like protein ENSP00000290377)
Product Synonym Names
Anti -YI016, CT (Putative tubulin beta chain-like protein ENSP00000290377)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
YI016 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-288 amino acids from the C-terminal region of human YI016.

Product Categories/Family for anti-YI016 antibody

Applications Tested/Suitable for anti-YI016 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-YI016 antibody
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunofluorescence: 1:10-50
Flow Cytometry: 1:10-50

Immunohistochemistry (IHC) of anti-YI016 antibody

YI016 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of YI016 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-YI016 Polyclonal Antibody – Cat# MBS646784

Western Blot (WB) of anti-YI016 antibody

YI016 Antibody (C-term) western blot analysis in K562,HL-60,NCI-H460 cell line lysates (35ug/lane).This demonstrates the YI016 antibody detected the YI016 protein (arrow).
Rabbit Anti-YI016 Polyclonal Antibody, Western Blot (WB) – Cat# MBS646784

Immunofluorescence (IF) of anti-YI016 antibody

Confocal immunofluorescent analysis of YI016 Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-YI016 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS646784

Flow Cytometry (FC/FACS) of anti-YI016 antibody

YI016 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-YI016 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS646784

Other Notes

Small volumes of anti-YI016 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for YI016 purchase

MBS646784 is an antibody targeting YI016. Also known as YI016, CT (Putative tubulin beta chain-like protein ENSP00000290377). Reactive in Human. Host: Rabbit.
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