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PRKD3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS646820

Protein kinase D (PKD), a serine/threonine kinase originally described as a novel PKC family member and termed PKCm, belongs to the calcium calmodulin superfamily of kinases. Three mammalian isoforms have so far been described - PKD1/PKCm, PKD2 and PKD3/PKCn;… More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PRKD3, Monoclonal Antibody
Full Product Name
PRKD3 (Serine/Threonine-protein Kinase D3, Protein Kinase C nu Type, Protein Kinase EPK2, nPKC-nu, EPK2, PRKCN)
Product Gene Name
anti-PRKD3 antibody
Product Synonym Names
Anti -PRKD3 (Serine/Threonine-protein Kinase D3, Protein Kinase C nu Type, Protein Kinase EPK2, nPKC-nu, EPK2, PRKCN)
Immunogen Sequence
MSANNSPPSA QKSVLPTAIP AVLPAASPCS SPKTGLSARL SNGSFSAPSL TNSRGSVHTV SFLLQIGLTR ESVTIEAQEL SLSAVKDLVC SIVYQKFPEC
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4G7
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human PRKD3.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
100,471 Da
Chromosome Location
Chromosome: 2; NC_000002.11 (37477645..37551915, complement). Location: 2p21
OMIM
607077
3D Structure
ModBase 3D Structure for O94806
Immunogen
Partial recombinant corresponding to aa1-100 from PRKD3 (AAH30706) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-PRKD3 antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-PRKD3 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-PRKD3 antibody
Suitable for use in ELISA and Western Blot.

Western Blot (WB) of anti-PRKD3 antibody

Western Blot detection against Immunogen (37kD).
Mouse Anti-PRKD3 Monoclonal Antibody – Cat# MBS646820

Testing Data of anti-PRKD3 antibody

Detection limit for recombinant GST tagged PRKD3 is ~1ng/ml as a capture antibody.
Mouse Anti-PRKD3 Monoclonal Antibody, Testing Data – Cat# MBS646820

Other Notes

Small volumes of anti-PRKD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRKD3 purchase

MBS646820 is an antibody targeting PRKD3. Also known as PRKD3 (Serine/Threonine-protein Kinase D3, Protein Kinase C nu Type, Protein Kinase EPK2, nPKC-nu, EPK2, PRKCN). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PRKD3. It may not necessarily be applicable to this product.

NCBI GI #
22658307
NCBI GeneID
23683
NCBI Accession #
AAH30706.1
UniProt Primary Accession #
O94806
UniProt Secondary Accession #
Q53TR7; Q8NEL8; D6W587
UniProt Related Accession #
O94806
NCBI Official Full Name
PRKD3 protein
NCBI Official Synonym Full Names
protein kinase D3
NCBI Official Symbol
PRKD3
NCBI Official Synonym Symbols
EPK2; PKD3; PRKCN; PKC-NU; nPKC-NU
UniProt Protein Name
Serine/threonine-protein kinase D3
UniProt Synonym Protein Names
Protein kinase C nu type; Protein kinase EPK2; nPKC-nu
Protein Family
Serine/threonine-protein kinase
UniProt Gene Name
PRKD3
UniProt Synonym Gene Names
EPK2; PRKCN
UniProt Entry Name
KPCD3_HUMAN
NCBI Protein Information
serine/threonine-protein kinase D3; protein kinase EPK2; protein kinase C, nu; protein kinase C nu type; protein-serine/threonine kinase

NCBI Summary for PRKD3

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members. [provided by RefSeq, Jul 2008]
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UniProt Comments for PRKD3

Function: Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress

By similarity.

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium

By similarity.

Enzyme regulation: Activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains 1 and 2 bind both DAG and phorbol ester with high affinity and mediate translocation to the cell membrane. Autophosphorylation of Ser-735 and phosphorylation of Ser-731 by PKC relieves auto-inhibition by the PH domain. Ref.7

Subcellular location: Cytoplasm. Membrane. Note: Translocation to the cell membrane is required for kinase activation. Ref.7

Tissue specificity: Ubiquitous.

Sequence similarities: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.Contains 1 PH domain.Contains 2 phorbol-ester/DAG-type zinc fingers.Contains 1 protein kinase domain.
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Research Articles on PRKD3

1. PKD3 contributes to the proliferation and malignant growth of androgen-dependent prostate cancer cells in part by upregulating PSA expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PRKD3 antibody

 Products by Pathway  Pathway Diagram
 G Protein Signaling Pathways antibodies  G Protein Signaling Pathways Diagram
 IL-4 Signaling Pathway antibodies  IL-4 Signaling Pathway Diagram
 MiRs In Muscle Cell Differentiation Pathway antibodies  MiRs In Muscle Cell Differentiation Pathway Diagram

Diseases associated with anti-PRKD3 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >3 publications with PRKD3 and Neoplasms
 Carcinoma Antibodies  >1 publications with PRKD3 and Carcinoma
 Lung Neoplasms Antibodies  >1 publications with PRKD3 and Lung Neoplasms

Organs/Tissues associated with anti-PRKD3 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >1 publications with PRKD3 and Lung
 Bone Antibodies  >1 publications with PRKD3 and Bone
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