• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

SYNJ2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS646824

MBS646824 is an antibody targeting SYNJ2. Also known as SYNJ2 (Synaptojanin-2, Synaptic Inositol 1,4,5-trisphosphate 5-phosphatase 2, INPP5H, KIAA0348). Reactive in Human. Host: Mouse. More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SYNJ2, Monoclonal Antibody
Full Product Name
SYNJ2 (Synaptojanin-2, Synaptic Inositol 1,4,5-trisphosphate 5-phosphatase 2, INPP5H, KIAA0348)
Product Gene Name
anti-SYNJ2 antibody
Product Synonym Names
Anti -SYNJ2 (Synaptojanin-2, Synaptic Inositol 1,4,5-trisphosphate 5-phosphatase 2, INPP5H, KIAA0348)
Immunogen Sequence
GTKAMKPEAA PLLGDYQDPF WNLLHHPKLL NNTWLSKSSD PLDSGTRSPK RDPIDPVSAG ASAAKAELPP DHEHKTLGHW VTISDQEKRT ALQVFDPLAK
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2H8
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human SYNJ2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
165,538 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (158402888..158520208). Location: 6q25.3
OMIM
609410
3D Structure
ModBase 3D Structure for O15056
Immunogen
Partial recombinant corresponding to aa1396-1496 from SYNJ2 (NP_003889) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-SYNJ2 antibody

Antibodies; Abs to Enzymes; Phosphatase
Applications Tested/Suitable for anti-SYNJ2 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-SYNJ2 antibody
Suitable for use in ELISA and Western Blot.

Western Blot (WB) of anti-SYNJ2 antibody

Western Blot analysis of SYNJ2 expression in transfected 293T cell line by SYNJ2 monoclonal antibody |Lane 1: SYNJ2 transfected lysate (92.2kD).|Lane 2: Non-transfected lysate.
Mouse Anti-SYNJ2 Monoclonal Antibody – Cat# MBS646824

Western Blot (WB) of anti-SYNJ2 antibody

Western Blot detection against Immunogen (37.11kD).
Mouse Anti-SYNJ2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS646824

Western Blot (WB) of anti-SYNJ2 antibody

Western blot analysis of SYNJ2 over-expressed 293 cell line, cotransfected with SYNJ2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SYNJ2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-SYNJ2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS646824

Other Notes

Small volumes of anti-SYNJ2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SYNJ2. It may not necessarily be applicable to this product.

NCBI GI #
296010889
NCBI GeneID
8871
NCBI Accession #
NP_001171559.1
UniProt Primary Accession #
O15056
UniProt Secondary Accession #
Q5TA13; Q5TA16; Q5TA19; Q86XK0; Q8IZA8; Q9H226
UniProt Related Accession #
O15056
GenBank Nucleotide #
NM_001178088.1
NCBI Official Full Name
synaptojanin-2 isoform 2
NCBI Official Synonym Full Names
synaptojanin 2
NCBI Official Symbol
SYNJ2
NCBI Official Synonym Symbols
INPP5H
UniProt Protein Name
Synaptojanin-2
UniProt Synonym Protein Names
Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 2
Protein Family
Synaptojanin
UniProt Gene Name
SYNJ2
UniProt Synonym Gene Names
KIAA0348
UniProt Entry Name
SYNJ2_HUMAN
NCBI Protein Information
synaptojanin-2; inositol phosphate 5'-phosphatase 2; synaptic inositol 1,4,5-trisphosphate 5-phosphatase 2; synaptic inositol-1,4,5-trisphosphate 5-phosphatase 2

NCBI Summary for SYNJ2

The gene is a member of the inositol-polyphosphate 5-phosphatase family. The encoded protein interacts with the ras-related C3 botulinum toxin substrate 1, which causes translocation of the encoded protein to the plasma membrane where it inhibits clathrin-mediated endocytosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
Show more

UniProt Comments for SYNJ2

Function: Inositol 5-phosphatase which may be involved in distinct membrane trafficking and signal transduction pathways. May mediate the inhibitory effect of Rac1 on endocytosis.

Catalytic activity: 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.

Subunit structure: Binds to GRB2. Isoform 2A binds to SYNJ2BP/OMP25. Isoform 2B2 C-terminal proline-rich region binds to a variety of SH3 domain-containing proteins including SH3GL1, SH3GL2, SH3GL3 and GRB2.

Subcellular location: Membrane

By similarity. Note: Predominantly associated with the particulate fractions

By similarity. Ref.2

Sequence similarities: Belongs to the synaptojanin family.In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.Contains 1 RRM (RNA recognition motif) domain.Contains 1 SAC domain.

Sequence caution: The sequence AAH43277.1 differs from that shown. Reason: Erroneous initiation.
Show more

Research Articles on SYNJ2

1. Src-mediated phosphorylation of SYNJ2 contributes to invadopodia formation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SYNJ2 antibody

 Products by Pathway  Pathway Diagram
 1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway antibodies  1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway Diagram
 1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway antibodies  1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway Diagram
 3-phosphoinositide Degradation Pathway antibodies  3-phosphoinositide Degradation Pathway Diagram
 3-phosphoinositide Degradation Pathway antibodies  3-phosphoinositide Degradation Pathway Diagram
 D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway antibodies  D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway Diagram
 D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway antibodies  D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway Diagram
 D-myo-inositol (1,4,5)-trisphosphate Degradation Pathway antibodies  D-myo-inositol (1,4,5)-trisphosphate Degradation Pathway Diagram
 D-myo-inositol (1,4,5)-trisphosphate Degradation Pathway antibodies  D-myo-inositol (1,4,5)-trisphosphate Degradation Pathway Diagram
 Inositol Phosphate Metabolism Pathway antibodies  Inositol Phosphate Metabolism Pathway Diagram
 Inositol Phosphate Metabolism Pathway antibodies  Inositol Phosphate Metabolism Pathway Diagram

Diseases associated with anti-SYNJ2 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2 publications with SYNJ2 and Nervous System Diseases
 Neoplasms Antibodies  >2 publications with SYNJ2 and Neoplasms
 Prostatic Neoplasms Antibodies  >1 publications with SYNJ2 and Prostatic Neoplasms
 Prostatic Diseases Antibodies  >1 publications with SYNJ2 and Prostatic Diseases

Organs/Tissues associated with anti-SYNJ2 antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >4 publications with SYNJ2 and Nerve
 Brain Antibodies  >4 publications with SYNJ2 and Brain
 Ganglia Antibodies  >1 publications with SYNJ2 and Ganglia
 Prostate Antibodies  >1 publications with SYNJ2 and Prostate
 Ear Antibodies  >1 publications with SYNJ2 and Ear
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000