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CDC25B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6468436

MBS6468436 is an antibody targeting CDC25B. Also known as CDC25B, Phosphorylated (Ser187) (M-phase Inducer Phosphatase 2, Dual Specificity Phosphatase Cdc25B, CDC25HU2) (Biotin). Reactive in Human. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CDC25B, Polyclonal Antibody
Full Product Name
CDC25B, Phosphorylated (Ser187) (M-phase Inducer Phosphatase 2, Dual Specificity Phosphatase Cdc25B, CDC25HU2) (Biotin)
Product Gene Name
anti-CDC25B antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Immunoaffinity chromatography.
Specificity
Recognizes human Cdc25B phosphorylated at Ser187.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
64.987
OMIM
116949
3D Structure
ModBase 3D Structure for P30305
Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser187 of human Cdc25B (KLH).
Conjugate
Biotin
Antigen Modification
Phosphorylated
Note
Preservative Free

Product Categories/Family for anti-CDC25B antibody

Antibodies; Protein Kinases
Applications Tested/Suitable for anti-CDC25B antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-CDC25B antibody
IHC: 1:50-1:100
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-CDC25B antibody

The anti-Phospho-CDC25B-S187 Pab is used in Western blot to detect Phospho-CDC25B-S187 in SK-BR-3 tissue lysate
Rabbit Anti-CDC25B Polyclonal Antibody – Cat# MBS6468436

Immunohistochemistry (IHC) of anti-CDC25B antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-CDC25B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6468436

Immunohistochemistry (IHC) of anti-CDC25B antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-CDC25B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6468436

Product References and Citations for anti-CDC25B antibody

1. Uchida, S., et al., Biochem. Biophys. Res. Commun. 316(1):226-232 (2004). 2. Ito, Y., et al., Int. J. Mol. Med. 13(3):431-435 (2004). 3. Wu, W., et al., Cancer Res. 63(19):6195-6199 (2003). 4. Mils, V., et al., Exp. Cell Res. 285(1):99-106 (2003). 5. Theis-Febvre, N., et al., Oncogene 22(2):220-232 (2003).

Other Notes

Small volumes of anti-CDC25B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CDC25B. It may not necessarily be applicable to this product.

NCBI GI #
11641415
NCBI Accession #
NP_068660
UniProt Primary Accession #
P30305
UniProt Protein Name
M-phase inducer phosphatase 2
UniProt Synonym Protein Names
Dual specificity phosphatase Cdc25B
UniProt Gene Name
CDC25B
UniProt Synonym Gene Names
CDC25HU2
UniProt Entry Name
MPIP2_HUMAN

UniProt Comments for CDC25B

CDC25B: a member of the MPI phosphatase family. Activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. Shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. At least four transcript variants for this gene exist. Three splice variant isoforms have been described.

Protein type: EC 3.1.3.48; Nuclear receptor co-regulator; Protein phosphatase, dual-specificity; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: nucleoplasm; spindle pole; centrosome; cytoplasm; cytosol

Molecular Function: protein binding; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: positive regulation of cytokinesis; mitosis; cell division; positive regulation of protein kinase activity; positive regulation of cell proliferation; mitotic cell cycle; G2/M transition of mitotic cell cycle; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; oocyte maturation; female meiosis I
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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