ARID5B Antibody – Rabbit Polyclonal

This gene encodes a member of the AT-rich interaction domain (ARID) family of DNA binding proteins. The encoded protein forms a histone H3K9Me2 demethylase complex with PHD finger protein 2 and regulates the transcription of target genes involved in adipogenesis and liver development. This gene also plays a role in cell growth and differentiation of B-lymphocyte progenitors, and single nucleotide polymorphisms in this gene are associated with acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]

Function: Transcription coactivator that binds to the 5'-AATA[CT]-3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation. Represses the cytomegalovirus enhancer. Ref.16

Subcellular location: Nucleus

By similarity.

Tissue specificity: Widely expressed, including in liver (at protein level). Ref.16

Domain: The ARID domain mediates the interaction with DNA.

Post-translational modification: Methylation at Lys-336 prevents DNA-binding. Demethylation by PHF2 promotes recruitment of the PHF2-ARID5B complex to promoters. Ref.16

Involvement in disease: Defects in ARID5B may be a cause of susceptibility to coronary atherosclerosis in the Japanese population.Leukemia, acute lymphoblastic (ALL) [MIM:613065]: A subtype of acute leukemia, a cancer of the white blood cells. ALL is a malignant disease of bone marrow and the most common malignancy diagnosed in children. The malignant cells are lymphoid precursor cells (lymphoblasts) that are arrested in an early stage of development. The lymphoblasts replace the normal marrow elements, resulting in a marked decrease in the production of normal blood cells. Consequently, anemia, thrombocytopenia, and neutropenia occur to varying degrees. The lymphoblasts also proliferate in organs other than the marrow, particularly the liver, spleen, and lymphonodes.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15

Sequence similarities: Belongs to the ARID5B family.Contains 1 ARID domain.

Sequence caution: The sequence AAH15120.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.The sequence AAH36831.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence AAH66345.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.The sequence AAI07801.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.The sequence BAB15012.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.