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FBXO3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS646919

FBXO3 encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
FBXO3, Polyclonal Antibody
Full Product Name
FBXO3, CT (FBXO3, FBX3, F-box only protein 3)
Product Gene Name
anti-FBXO3 antibody
Product Synonym Names
Anti -FBXO3, CT (FBXO3, FBX3, F-box only protein 3)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
54,561 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (33762490..33796071, complement). Location: 11p13
OMIM
609089
3D Structure
ModBase 3D Structure for Q9UK99
Immunogen
FBXO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401~431 amino acids from the C-terminal region of human FBXO3.

Product Categories/Family for anti-FBXO3 antibody

Antibodies; Abs to Ubiquitin Ligases
Applications Tested/Suitable for anti-FBXO3 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-FBXO3 antibody
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-FBXO3 antibody

Western blot analysis of HepG2 cell line lysates (35ug/lane) using MBS646919. FBXO3 (arrow) was detected using the purified Pab.
Rabbit Anti-FBXO3 Polyclonal Antibody – Cat# MBS646919

Flow Cytometry (FC/FACS) of anti-FBXO3 antibody

Flow Cytometry analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram) using MBS646919. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-FBXO3 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS646919

Other Notes

Small volumes of anti-FBXO3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FBXO3 purchase

MBS646919 is an antibody targeting FBXO3. Also known as FBXO3, CT (FBXO3, FBX3, F-box only protein 3). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FBXO3. It may not necessarily be applicable to this product.

NCBI GI #
71297179
NCBI GeneID
26273
NCBI Accession #
AAH39291.1
UniProt Primary Accession #
Q9UK99
UniProt Secondary Accession #
Q86X90; Q9H0V2; Q9NUX2; B3KY16; D3DR05
UniProt Related Accession #
Q9UK99
NCBI Official Full Name
FBXO3 protein
NCBI Official Synonym Full Names
F-box protein 3
NCBI Official Symbol
FBXO3
NCBI Official Synonym Symbols
FBA; FBX3
UniProt Protein Name
F-box only protein 3
Protein Family
F-box only protein
UniProt Gene Name
FBXO3
UniProt Synonym Gene Names
FBX3
UniProt Entry Name
FBX3_HUMAN
NCBI Protein Information
F-box only protein 3; F-box protein FBX3

NCBI Summary for FBXO3

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants diverging at the 3' end. [provided by RefSeq, Jul 2008]
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UniProt Comments for FBXO3

Function: Substrate recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation. Ref.6

Subunit structure: Part of a SCF (SKP1-cullin-F-box) protein ligase complex consisting of FBXO3, SKP1, CUL1 and RBX1. Interacts with PML, interaction is direct and takes place either alone or within the SCF complex. Ref.6

Subcellular location: Nucleus. Note: Colocalizes with PML at the peripheries of nuclear bodies.

Sequence similarities: Contains 1 apaG domain.Contains 1 F-box domain.
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Research Articles on FBXO3

1. controls TRAF adaptor stability to regulate inflammation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-FBXO3 antibody

 Reference Product  PubMed Publications
 HIPK2 antibody  >1 publications with FBXO3 and HIPK2
 SKP1 antibody  >1 publications with FBXO3 and SKP1

Pathways associated with anti-FBXO3 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram

Diseases associated with anti-FBXO3 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2 publications with FBXO3 and Neoplasms
 Inflammation Antibodies  >2 publications with FBXO3 and Inflammation
 Chromosome Aberrations Antibodies  >1 publications with FBXO3 and Chromosome Aberrations
 Carcinoma, Squamous Cell Antibodies  >1 publications with FBXO3 and Carcinoma, Squamous Cell
 Necrosis Antibodies  >1 publications with FBXO3 and Necrosis

Organs/Tissues associated with anti-FBXO3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1 publications with FBXO3 and Blood
 Lung Antibodies  >1 publications with FBXO3 and Lung
 Intestine Antibodies  >1 publications with FBXO3 and Intestine
 Mouth Antibodies  >1 publications with FBXO3 and Mouth
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