Caspase 3, Pro, Active/Cleaved (CASP5) Antibody – Rabbit Polyclonal

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein; this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]

Function: Mediator of programmed cell death (apoptosis).

Catalytic activity: Strict requirement for Asp at the P1 position. It has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|- but also cleaves at Asp-Glu-Val-Asp-|-.

Subunit structure: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunits.

Tissue specificity: Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.

Induction: Up-regulated by bacterial lipopolysaccharides (LPS). Ref.1

Post-translational modification: The two subunits are derived from the precursor sequence by an autocatalytic mechanism.

Sequence similarities: Belongs to the peptidase C14A family.Contains 1 CARD domain.

Sequence caution: The sequence AAA75172.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAH74994.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAI13407.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence ABF47103.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAG59257.1 differs from that shown. Reason: Frameshift at position 10. The sequence CAA64450.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.