FBXL2 Antibody – Rabbit Polyclonal

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Function: Calcium-activated substrate recognition component of a SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 doesn't seem to target phosphodegron within its substrates but rather calmodulin-binding motifs. Targets PCYT1A for its monoubiquitination and degradation, this is antagonized by calmodulin

By similarity. Targets the cyclins CCND2 and CCND3 for polyubiquitination and degradation, leading to cell-cycle arrest in G0, also antagonized by calmodulin. Binds to hepatitis C virus non-structural protein 5A (NS5A) in a reaction crucial for hepatitis C virus RNA replication. Ref.9 Ref.10 Ref.11

Subunit structure: Interacts with SKP1. Interacts with PCYT1A. Part of a SCF (SKP1-cullin-F-box) protein ligase complex

By similarity. Interacts (via CAAX motif) with hepatitis C virus non-structural protein 5A (NS5A). May also interact, but less efficiently, with hepatitis C virus non-structural protein 5B (NS5B). Interacts with CCND2 and CCND3. Ref.2 Ref.9 Ref.10 Ref.11

Subcellular location: Membrane; Lipid-anchor Ref.9.

Tissue specificity: Expressed in brain, heart, kidney, liver, lung, pancreas and placenta. Ref.2

Miscellaneous: Deletion of the F-box domain creates a dominant-negative protein that inhibits replication of hepatitis C virus RNA when overexpressed in a hepatoma cell line; this inhibition could be overcome by NS5A coexpression.

Sequence similarities: Contains 1 F-box domain.Contains 13 LRR (leucine-rich) repeats.

Sequence caution: The sequence AAF03128.1 differs from that shown. Reason: Erroneous initiation.