Rabbit Anti-QTRT1 Polyclonal Antibody – Cat# MBS647000

QTRT1 Antibody (C-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the QTRT1 antibody detected the QTRT1 protein (arrow).

QTRT1 Antibody – Rabbit Polyclonal

Name: QTRT1 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS647000
Price: 895 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647000

tRNA-guanine transglycosylase (TGT; EC 2.4.2.29) synthesizes queuosine (Q), which is found in tRNAs that recognize NAU and NAC codons, encoding tyr, asn, asp, and his. Prokaryotic TGT is a single protein of 43 kD. In contrast, mammalian TGT appears to be a… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

QTRT1, Polyclonal Antibody

Full Product Name

QTRT1, CT (QTRT1, TGT, TGUT, Queuine tRNA-ribosyltransferase, Guanine insertion enzyme, tRNA-guanine transglycosylase)

Product Gene Name

anti-QTRT1 antibody

Product Synonym Names

Anti -QTRT1, CT (QTRT1, TGT, TGUT, Queuine tRNA-ribosyltransferase, Guanine insertion enzyme, tRNA-guanine transglycosylase)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Human

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

44,048 Da

Chromosome Location

Chromosome: 19; NC_000019.9 (10812112..10824043). Location: 19p13.3

OMIM

609615

3D Structure

ModBase 3D Structure for Q9BXR0

Immunogen

QTRT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 344-371 amino acids from the C-terminal region of human QTRT1.

Product Categories/Family for anti-QTRT1 antibody

Antibodies; Abs to Enzymes; Ribosyltransferase

Applications Tested/Suitable for anti-QTRT1 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-QTRT1 antibody

Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Other Notes

Small volumes of anti-QTRT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for QTRT1 purchase

MBS647000 is an antibody targeting QTRT1. Also known as QTRT1, CT (QTRT1, TGT, TGUT, Queuine tRNA-ribosyltransferase, Guanine insertion enzyme, tRNA-guanine transglycosylase). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for QTRT1. It may not necessarily be applicable to this product.

NCBI GI #

13654276

NCBI GeneID

81890

NCBI Accession #

NP_112486.1

UniProt Primary Accession #

Q9BXR0

UniProt Secondary Accession #

Q96BQ4; Q9BXQ9

UniProt Related Accession #

Q9BXR0

GenBank Nucleotide #

NM_031209.2

NCBI Official Full Name

queuine tRNA-ribosyltransferase

NCBI Official Synonym Full Names

queuine tRNA-ribosyltransferase 1

NCBI Official Symbol

QTRT1

NCBI Official Synonym Symbols

TGT; TGUT; FP3235

UniProt Protein Name

Queuine tRNA-ribosyltransferase

UniProt Synonym Protein Names

Guanine insertion enzyme; tRNA-guanine transglycosylase

Protein Family

Queuine tRNA-ribosyltransferase

UniProt Gene Name

QTRT1

UniProt Synonym Gene Names

TGT; TGUT

UniProt Entry Name

TGT_HUMAN

NCBI Protein Information

queuine tRNA-ribosyltransferase; TGT, 43-KD subunit; TGT, catalytic subunit; guanine insertion enzyme; tRNA-guanine transglycosylase

NCBI Summary for QTRT1

This gene encodes the catalytic subunit of tRNA-guanine transglycosylase. tRNA-guanine transglycosylase is a heterodimeric enzyme complex that plays a critical role in tRNA modification by synthesizing the 7-deazaguanosine queuosine, which is found in tRNAs that code for asparagine, aspartic acid, histidine and tyrosine. A pseudogene of this gene is located on the long arm of chromosome X. [provided by RefSeq, Feb 2012]

UniProt Comments for QTRT1

Function: Interacts with QTRTD1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine)

By similarity.

Catalytic activity: Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine.

Cofactor: Binds 1 zinc ion per subunit

By similarity.

Pathway: tRNA modification; tRNA-queuosine biosynthesis.

Subunit structure: Interacts with QTRTD1

By similarity.

Subcellular location: Cytoplasm

By similarity. Mitochondrion

By similarity. Nucleus

By similarity. Note: Weakly associates with mitochondria, possibly via QTRTD1

By similarity.

Sequence similarities: Belongs to the queuine tRNA-ribosyltransferase family.

Sequence caution: The sequence AAH15350.1 differs from that shown. Reason: Erroneous initiation.

Research Articles on QTRT1

1. the inhibition of the human TGT by biopterin, consistent with earlier reports on other eukaryal TGTs, and supportive of the concept that pteridines may regulate eukaryal TGT activity in vivo.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-QTRT1 antibody

Reference Product	PubMed Publications
USP14 antibody	>1 publications with QTRT1 and USP14