Goat Anti-DBNL Polyclonal Antibody – Cat# MBS647041

staining (1ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

HIP-55 (DBNL) Antibody – Goat Polyclonal

Name: DBNL Antibody; Polyclonal; Goat; MyBioSource
Brand: MyBioSource
SKU: MBS647041
Price: 835 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647041

DBNL is an F-actin-binding, cytoskeleton adapter protein, and member of the ABP1 family, implicated in the connection of the actin cytoskeleton to endocytic function, through association with the GTPase dynamin. DBNL is degraded in a caspase-dependent manner… More details

$835 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

HIP-55 (DBNL), Polyclonal Antibody

Full Product Name

HIP-55 (Drebrin-like Protein, Cervical SH3P7, Cervical Mucin-associated Protein, Drebrin-F, HPK1-interacting Protein of 55kD, SH3 Domain-containing Protein 7, DBNL, CMAP, SH3P7, PP5423)

Product Gene Name

anti-DBNL antibody

Product Synonym Names

Anti -HIP-55 (Drebrin-like Protein, Cervical SH3P7, Cervical Mucin-associated Protein, Drebrin-F, HPK1-interacting Protein of 55kD, SH3 Domain-containing Protein 7, DBNL, CMAP, SH3P7, PP5423)

Clonality

Polyclonal

Host

Goat

Species Reactivity

Canine, Human, Mouse, Rat

Form/Format

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes DBNL. Species Crossreactivity

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

48,207 Da

Chromosome Location

Chromosome: 7; NC_000007.13 (44084239..44101315). Location: 7p13

OMIM

610106

3D Structure

ModBase 3D Structure for Q9UJU6

Immunogen

Synthetic peptide corresponding to AANLSRNGPALQE-C, from DBNL, at N-terminal (NP_054782.2, NP_001014436.1, NP_001116428.1).

Product Categories/Family for anti-DBNL antibody

Antibodies; Abs to Actin Related Proteins

Applications Tested/Suitable for anti-DBNL antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-DBNL antibody

Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:32,000
Western Blot: 1-3ug/ml, observed in Jurkat and 3T3 lysates on ~55kD bands

Other Notes

Small volumes of anti-DBNL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DBNL purchase

MBS647041 is an antibody targeting DBNL. Also known as HIP-55 (Drebrin-like Protein, Cervical SH3P7, Cervical Mucin-associated Protein, Drebrin-F, HPK1-interacting Protein of 55kD, SH3 Domain-containing Protein 7, DBNL, CMAP, SH3P7, PP5423). Reactive in Canine, Human, Mouse, Rat. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for DBNL. It may not necessarily be applicable to this product.

NCBI GI #

48146451

NCBI GeneID

28988

NCBI Accession #

CAG33448.1

UniProt Primary Accession #

Q9UJU6

UniProt Secondary Accession #

P84070; Q6IAI8; Q96F30; Q96K74; Q9HBN8; Q9NR72; A4D2I9

UniProt Related Accession #

Q9UJU6

NCBI Official Full Name

HIP-55

NCBI Official Synonym Full Names

drebrin-like

NCBI Official Symbol

DBNL

NCBI Official Synonym Symbols

ABP1; HIP55; SH3P7; HIP-55

UniProt Protein Name

Drebrin-like protein

UniProt Synonym Protein Names

Cervical SH3P7; Cervical mucin-associated protein; Drebrin-F; HPK1-interacting protein of 55 kDa; HIP-55; SH3 domain-containing protein 7

Protein Family

Drebrin-like protein

UniProt Gene Name

DBNL

UniProt Synonym Gene Names

CMAP; SH3P7; HIP-55

UniProt Entry Name

DBNL_HUMAN

NCBI Protein Information

drebrin-like protein; drebrin-F; cervical SH3P7; SH3 domain-containing protein 7; cervical mucin-associated protein; HPK1-interacting protein of 55 kDa; src homology 3 domain-containing protein HIP-55

UniProt Comments for DBNL

Function: Adapter protein that binds F-actin and DNM1, and thereby plays a role in receptor-mediated endocytosis. Plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. Does not bind G-actin and promote actin polymerization by itself. Required for the formation of organized podosome rosettes

By similarity. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Ref.13

Subunit structure: Interacts with SHANK2, SHANK3 and SYN1. Interacts with FGD1 and DNM1. Interacts with ANKRD54. Interacts with COBL. Interacts with WASL and WIPF1

By similarity. Interacts with MAP4K1 and PRAM1. Ref.1 Ref.14

Subcellular location: Cytoplasm › cytoskeleton. Cell projection › lamellipodium. Cell projection › ruffle

By similarity. Cytoplasm › cell cortex

By similarity. Cytoplasm › cytosol

By similarity. Cell junction › synapse

By similarity. Cell projection

By similarity. Cell membrane; Peripheral membrane protein; Cytoplasmic side

By similarity. Cytoplasmic vesicle › clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side

By similarity. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side

By similarity. Cell projection › podosome

By similarity. Note: Detected in neuron cell body and cell projections, such as neurites. Colocalizes with cytosolic dynamin in hippocampus neurons

By similarity. Cortical actin cytoskeleton. Associates with lamellipodial actin and membrane ruffles. Colocalizes with actin and cortactin at podosome dots and podosome rosettes

By similarity.

Domain: The SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1.

Post-translational modification: Degraded by caspases during apoptosis.

Sequence similarities: Belongs to the ABP1 family.Contains 1 ADF-H domain.Contains 1 SH3 domain.

Research Articles on DBNL

1. mAbp1 is of fundamental importance for spreading and migration under shear stress conditions, which are critical prerequisites for efficient PMN extravasation during inflammation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-DBNL antibody

Products by Pathway	Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptotic Cleavage Of Cellular Proteins Pathway antibodies	Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
Apoptotic Execution Phase Pathway antibodies	Apoptotic Execution Phase Pathway Diagram
Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway antibodies	Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway Diagram
JNK Signaling In The CD4+ TCR Pathway antibodies	JNK Signaling In The CD4+ TCR Pathway Diagram
TCR Signaling Pathway antibodies	TCR Signaling Pathway Diagram
TCR Signaling In Naive CD4+ T Cells Pathway antibodies	TCR Signaling In Naive CD4+ T Cells Pathway Diagram

Diseases associated with anti-DBNL antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>2 publications with DBNL and Neoplasms
Lymphoma Antibodies	>1 publications with DBNL and Lymphoma
Nervous System Diseases Antibodies	>1 publications with DBNL and Nervous System Diseases
Breast Neoplasms Antibodies	>1 publications with DBNL and Breast Neoplasms
Hypertension Antibodies	>1 publications with DBNL and Hypertension
Cardiovascular Diseases Antibodies	>1 publications with DBNL and Cardiovascular Diseases
Disease Models, Animal Antibodies	>1 publications with DBNL and Disease Models, Animal
Necrosis Antibodies	>1 publications with DBNL and Necrosis
Inflammation Antibodies	>1 publications with DBNL and Inflammation

Organs/Tissues associated with anti-DBNL antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>9 publications with DBNL and Brain
Muscle Antibodies	>4 publications with DBNL and Muscle
Blood Antibodies	>4 publications with DBNL and Blood
Spleen Antibodies	>2 publications with DBNL and Spleen
Lung Antibodies	>2 publications with DBNL and Lung
Pancreas Antibodies	>1 publications with DBNL and Pancreas
Embryonic Tissue Antibodies	>1 publications with DBNL and Embryonic Tissue
Prostate Antibodies	>1 publications with DBNL and Prostate
Vascular Antibodies	>1 publications with DBNL and Vascular
Thymus Antibodies	>1 publications with DBNL and Thymus