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CREB Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6470590

MBS6470590 is an antibody targeting CREB. Also known as CREB, phosphorylated, Ser133 (cAMP Response Element Binding Protein) (AP). Reactive in Rat, Hamster, Human, Mouse. Host: Rabbit. More details

$1205 / 0.1 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CREB, Polyclonal Antibody
Full Product Name
CREB, phosphorylated, Ser133 (cAMP Response Element Binding Protein) (AP)
Product Gene Name
anti-CREB antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Rat, Hamster, Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Purity/Purification
Purified by Dual Antigen affinity chromatography.
Specificity
Recognizes rat CREB when phosphorylated on Ser133, Mr ~43kD. May recognize ATF-1 and CREM due to signifigant homology to phosphorylated immunizing peptide. Species Crossreactivity: mouse, human and hamster
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
35,081 Da
3D Structure
ModBase 3D Structure for P15337
Immunogen
Synthetic peptide corresponding to amino acids including and emcompassing phosphorylated Ser133 of human CREB conjugated to KLH (P16220).
Conjugate
AP
Antigen Modification
Phosphorylated
Note
Preservative Free

Product Categories/Family for anti-CREB antibody

Antibodies; Transcription Factors
Applications Tested/Suitable for anti-CREB antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)
Application Notes for anti-CREB antibody
IHC-P: 1:1000 dilution detects phosphorylated CREB in formalin-fixed paraffin-embedded normal and ischemic rat brain sections.
WB: 1:1000 Detected phosphorylated CREB in RIPA lysates from mouse 3T3 cells treated with 50mM forskolin. PC-12 and forskolin treated PC-12 cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-CREB (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
IP: 10ul immunoprecipitates phosphorylated CREB from 500ug of rat PC-12 cells treated with 50uM forskolin
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-CREB antibody

WB: untreated (lane 1) and forskolin treated (lane 2) 3T3 cell lysates were probed with MBS611737 (1:1000).
Rabbit Anti-CREB Polyclonal Antibody – Cat# MBS6470590

Product References and Citations for anti-CREB antibody

1. Ginty, et al., (1993) Science 260: 238-241. 2. Lei, Z., and Rao, C.V, (1997). J. Biol. Chem. 272: 14365-14371, 1997. 3. Le Tulzo, Y., et al., (1997). J. Clin. Invest. 99: 1516-1524, 1997. 4. Wu, G. Y., et al. (2001). USA., 98: 2808-2813. 5. Samato, H., et al. (2003). 278: 28659-28667.

Other Notes

Small volumes of anti-CREB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CREB. It may not necessarily be applicable to this product.

NCBI GI #
13591924
NCBI GeneID
81646
NCBI Accession #
NP_112279.1
UniProt Primary Accession #
P15337
UniProt Related Accession #
P15337
GenBank Nucleotide #
NM_031017.1
NCBI Official Full Name
cyclic AMP-responsive element-binding protein 1 isoform B
NCBI Official Synonym Full Names
cAMP responsive element binding protein 1
NCBI Official Symbol
Creb1
NCBI Official Synonym Symbols
Creb
UniProt Protein Name
Cyclic AMP-responsive element-binding protein 1
Protein Family
Cyclic AMP-responsive element-binding protein
UniProt Gene Name
Creb1
UniProt Synonym Gene Names
Creb-1; CREB-1; cAMP-responsive element-binding protein 1
UniProt Entry Name
CREB1_RAT
NCBI Protein Information
cyclic AMP-responsive element-binding protein 1; CREB-1; Y protein; cAMP response element binding protein 1; cAMP-responsive element-binding protein 1

NCBI Summary for CREB

binds the cAMP response element in many gene promoters and regulates transcription [RGD, Feb 2006]

UniProt Comments for CREB

CREB: a transcription factor of the leucine zipper family of DNA binding proteins. Binds as a homodimer to the cAMP-responsive element (CRE). Phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Two splice-variant isoforms have been described.

Protein type: Transcription factor; DNA-binding

Cellular Component: nucleoplasm; transcription factor complex; mitochondrion; nuclear chromatin; chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; histone acetyltransferase binding; protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; double-stranded DNA binding; transcription factor activity; transcription factor binding

Biological Process: circadian rhythm; transcription from RNA polymerase II promoter; lactation; response to glucagon stimulus; positive regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; positive regulation of multicellular organism growth; protein amino acid phosphorylation; regulation of apoptosis; response to organic substance; regulation of transcription, DNA-dependent; mammary gland development; transforming growth factor beta receptor signaling pathway; positive regulation of transforming growth factor-beta3 production; positive regulation of transcriptional preinitiation complex assembly; cell differentiation; response to drug; protein stabilization; secretory granule organization and biogenesis; positive regulation of hormone secretion; positive regulation of lipid biosynthetic process; regulation of circadian rhythm; memory; axonogenesis; pituitary gland development; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of cell size
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Research Articles on CREB

1. Stimulation of long-term potentiation results in an increase in the levels of CREB protein in hypothyroidism.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CREB antibody

 Reference Product  PubMed Publications
 Fos antibody  >54 publications with CREB and Fos
 Mapk1 antibody  >38 publications with CREB and Mapk1
 Mapk3 antibody  >34 publications with CREB and Mapk3
 Crebbp antibody  >23 publications with CREB and Crebbp
 Ep300 antibody  >9 publications with CREB and Ep300
 Akt1 antibody  >9 publications with CREB and Akt1
 Camk4 antibody  >6 publications with CREB and Camk4
 Ciita antibody  >3 publications with CREB and Ciita
 Camk2b antibody  >2 publications with CREB and Camk2b
 Nr3c1 antibody  >1 publications with CREB and Nr3c1

Pathways associated with anti-CREB antibody

 Products by Pathway  Pathway Diagram
 AKT Phosphorylates Targets In The Nucleus Pathway antibodies  AKT Phosphorylates Targets In The Nucleus Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies  Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram

Diseases associated with anti-CREB antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >111 publications with CREB and Nervous System Diseases
 Inflammation Antibodies  >67 publications with CREB and Inflammation
 Necrosis Antibodies  >45 publications with CREB and Necrosis
 Depressive Disorder Antibodies  >43 publications with CREB and Depressive Disorder
 Memory Disorders Antibodies  >42 publications with CREB and Memory Disorders
 Neurobehavioral Manifestations Antibodies  >37 publications with CREB and Neurobehavioral Manifestations
 Learning Disorders Antibodies  >32 publications with CREB and Learning Disorders
 Lung Neoplasms Antibodies  >27 publications with CREB and Lung Neoplasms
 Atherosclerosis Antibodies  >21 publications with CREB and Atherosclerosis
 Liver Diseases Antibodies  >18 publications with CREB and Liver Diseases

Organs/Tissues associated with anti-CREB antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >300 publications with CREB and Brain
 Heart Antibodies  >35 publications with CREB and Heart
 Embryonic Tissue Antibodies  >25 publications with CREB and Embryonic Tissue
 Pancreas Antibodies  >16 publications with CREB and Pancreas
 Testis Antibodies  >14 publications with CREB and Testis
 Thymus Antibodies  >4 publications with CREB and Thymus
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