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PRIM2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647099

The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit,… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PRIM2, Polyclonal Antibody
Full Product Name
PRIM2, ID (PRIM2, PRIM2A, DNA primase large subunit, DNA primase 58kD subunit)
Product Gene Name
Product Synonym Names
Anti -PRIM2, ID (PRIM2, PRIM2A, DNA primase large subunit, DNA primase 58kD subunit)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
58,806 Da
Chromosome Location
6p12-p11.1
OMIM
176636
3D Structure
ModBase 3D Structure for P49643
Immunogen
PRIM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-345 amino acids from the Central region of human PRIM2.

Product Categories/Family for anti-PRIM2 antibody

Applications Tested/Suitable for anti-PRIM2 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-PRIM2 antibody
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Western Blot (WB) of anti-PRIM2 antibody

PRIM2 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PRIM2 antibody detected the PRIM2 protein (arrow).
Rabbit Anti-PRIM2 Polyclonal Antibody – Cat# MBS647099

Other Notes

Small volumes of anti-PRIM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRIM2 purchase

MBS647099 is an antibody targeting PRIM2. Also known as PRIM2, ID (PRIM2, PRIM2A, DNA primase large subunit, DNA primase 58kD subunit). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PRIM2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
PRIM2 protein
NCBI Official Synonym Full Names
primase, DNA, polypeptide 2 (58kDa)
NCBI Official Symbol
PRIM2
NCBI Official Synonym Symbols
p58; PRIM2A
UniProt Protein Name
DNA primase large subunit
UniProt Synonym Protein Names
DNA primase 58 kDa subunit
UniProt Gene Name
PRIM2
UniProt Synonym Gene Names
PRIM2A; p58
UniProt Entry Name
PRI2_HUMAN
NCBI Protein Information
DNA primase large subunit; DNA primase subunit p58; DNA primase 58 kDa subunit; primase polypeptide 2A, 58kDa

NCBI Summary for PRIM2

This gene encodes the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers that are used to create Okazaki fragments on the lagging strand of the DNA. [provided by RefSeq, Sep 2013]
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UniProt Comments for PRIM2

Function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.

Cofactor: Binds 1 4Fe-4S cluster. Ref.4

Subunit structure: Heterodimer of a small subunit and a large subunit.

Sequence similarities: Belongs to the eukaryotic-type primase large subunit family.
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Research Articles on PRIM2

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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