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ALDH4A1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647226

ALDH4A1 belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency… More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ALDH4A1, Monoclonal Antibody
Full Product Name
ALDH4A1 (Aldehyde Dehydrogenase Family 4 Member A1, Delta-1-pyrroline-5-carboxylate Dehydrogenase, Mitochondrial, P5C Dehydrogenase, ALDH4, P5CDH)
Product Gene Name
anti-ALDH4A1 antibody
Product Synonym Names
Anti -ALDH4A1 (Aldehyde Dehydrogenase Family 4 Member A1, Delta-1-pyrroline-5-carboxylate Dehydrogenase, Mitochondrial, P5C Dehydrogenase, ALDH4, P5CDH)
Immunogen Sequence
MLLPAPALRR ALLSRPWTGA GLRWKHTSSL KVANEPVLAF TQGSPERDAL QKALKDLKGR MEAIPCVVGD EEVWTSDVQY QVSPFNHGHK VAKFCYADKS LLNKAIEAAL AARKEWDLKP IADRAQIFLK AADMLSGPRR AEILAKTMVG QGKTVIQAEI DAAAELIDFF RFNAKYAVEL EGQQPISVPP STNSTVYRGL EGFVAAISPF NFTAIGGNLA GAPALMGNVV LWKPSDTAML ASYAVYRILR EAGLP
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1A12-A5
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human ALDH4A1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
61,495 Da
Chromosome Location
Chromosome: 2; AC_000159.1 (134345970..134380243). Location: chromosome: 2
3D Structure
ModBase 3D Structure for A7YWE4
Immunogen
Full length recombinant corresponding to aa1-564 from ALDH4A1 (AAH07581) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-ALDH4A1 antibody

Antibodies; Abs to Enzymes; Dehydrogenase
Applications Tested/Suitable for anti-ALDH4A1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ALDH4A1 antibody
Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml

Immunohistochemistry (IHC) of anti-ALDH4A1 antibody

Immunoperoxidase of monoclonal antibody to ALDH4A1 on formalin-fixed paraffin-embedded human liver tissue.[antibody concentration 5ug/ml].
Mouse Anti-ALDH4A1 Monoclonal Antibody – Cat# MBS647226

Testing Data of anti-ALDH4A1 antibody

Detection limit for recombinant GST tagged ALDH4A1 is 0.1ng/ml as a capture antibody.
Mouse Anti-ALDH4A1 Monoclonal Antibody, Testing Data – Cat# MBS647226

Western Blot (WB) of anti-ALDH4A1 antibody

Western Blot analysis of ALDH4A1 expression in transfected 293T cell line by ALDH4A1 monoclonal antibody |Lane 1: ALDH4A1 transfected lysate (61.7kD).|Lane 2: Non-transfected lysate.
Mouse Anti-ALDH4A1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS647226

Western Blot (WB) of anti-ALDH4A1 antibody

ALDH4A1 monoclonal antibody Western Blot analysis of ALDH4A1 expression in A-431.
Mouse Anti-ALDH4A1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS647226

Other Notes

Small volumes of anti-ALDH4A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ALDH4A1 purchase

MBS647226 is an antibody targeting ALDH4A1. Also known as ALDH4A1 (Aldehyde Dehydrogenase Family 4 Member A1, Delta-1-pyrroline-5-carboxylate Dehydrogenase, Mitochondrial, P5C Dehydrogenase, ALDH4, P5CDH). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ALDH4A1. It may not necessarily be applicable to this product.

NCBI GI #
157278942
NCBI GeneID
100126042
NCBI Accession #
AAI34525.1
UniProt Primary Accession #
A7YWE4
UniProt Related Accession #
A7YWE4
NCBI Official Full Name
ALDH4A1 protein
NCBI Official Synonym Full Names
aldehyde dehydrogenase 4 family, member A1
NCBI Official Symbol
ALDH4A1
UniProt Protein Name
Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
UniProt Synonym Protein Names
Aldehyde dehydrogenase family 4 member A1; L-glutamate gamma-semialdehyde dehydrogenase
UniProt Gene Name
ALDH4A1
UniProt Synonym Gene Names
P5C dehydrogenase
UniProt Entry Name
AL4A1_BOVIN
NCBI Protein Information
delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial; P5C dehydrogenase; aldehyde dehydrogenase family 4 member A1

UniProt Comments for ALDH4A1

Function: Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes

By similarity.

Catalytic activity: L-glutamate 5-semialdehyde + NAD+ + H2O = L-glutamate + NADH.

Pathway: Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.

Subunit structure: Homodimer

By similarity.

Subcellular location: Mitochondrion matrix

By similarity.

Sequence similarities: Belongs to the aldehyde dehydrogenase family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ALDH4A1 antibody

 Products by Pathway  Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Proline Catabolism Pathway antibodies  Proline Catabolism Pathway Diagram

Diseases associated with anti-ALDH4A1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2 publications with ALDH4A1 and Neoplasms
 Cell Transformation, Neoplastic Antibodies  >1 publications with ALDH4A1 and Cell Transformation, Neoplastic
 Atrophy Antibodies  >1 publications with ALDH4A1 and Atrophy
 Liver Neoplasms Antibodies  >1 publications with ALDH4A1 and Liver Neoplasms
 Fatty Liver Antibodies  >1 publications with ALDH4A1 and Fatty Liver
 Hyperprolinemia type 2 Antibodies  >1 publications with ALDH4A1 and Hyperprolinemia type 2

Organs/Tissues associated with anti-ALDH4A1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7 publications with ALDH4A1 and Blood
 Skin Antibodies  >4 publications with ALDH4A1 and Skin
 Liver Antibodies  >4 publications with ALDH4A1 and Liver
 Ovary Antibodies  >1 publications with ALDH4A1 and Ovary
 Brain Antibodies  >1 publications with ALDH4A1 and Brain
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