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VAMP4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647270

MBS647270 is an antibody targeting VAMP4. Also known as VAMP4, ID (VAMP4, Vesicle-associated membrane protein 4). Reactive in Human, Mouse. Host: Rabbit. More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
VAMP4, Polyclonal Antibody
Full Product Name
VAMP4, ID (VAMP4, Vesicle-associated membrane protein 4)
Product Gene Name
Product Synonym Names
Anti -VAMP4, ID (VAMP4, Vesicle-associated membrane protein 4)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Concentration
0.97mg/mL (lot specific)
Specificity
Recognizes human VAMP4. Species Crossreactivity: mouse
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
16,397 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (171669296..171711379, complement). Location: 1q24-q25
OMIM
606909
3D Structure
ModBase 3D Structure for O75379
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 16-45 from the central region of human VAMP4.
Applications Tested/Suitable for anti-VAMP4 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-VAMP4 antibody
Western Blot: 1:1000
Immunohistochemistry (FFPE): 1:10-1:50
Optimal dilutions to be determined by the researcher.
Other applications not tested.

Western Blot (WB) of anti-VAMP4 antibody

VAMP4 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the VAMP4 antibody detected the VAMP4 protein (arrow).
Rabbit Anti-VAMP4 Polyclonal Antibody – Cat# MBS647270

Western Blot (WB) of anti-VAMP4 antibody

VAMP4 Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the VAMP4 antibody detected the VAMP4 protein (arrow).
Rabbit Anti-VAMP4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS647270

Immunohistochemistry (IHC) of anti-VAMP4 antibody

Immunohistochemistry analysis of formalin fixed paraffin embedded human hepatocarcinoma using MBS647270 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS647270 for immunohistochemistry.
Rabbit Anti-VAMP4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS647270

Other Notes

Small volumes of anti-VAMP4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for VAMP4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
vesicle-associated membrane protein 4 isoform 1
NCBI Official Synonym Full Names
vesicle-associated membrane protein 4
NCBI Official Symbol
VAMP4
NCBI Official Synonym Symbols
VAMP-4; VAMP24
UniProt Protein Name
Vesicle-associated membrane protein 4
UniProt Gene Name
VAMP4
UniProt Synonym Gene Names
VAMP-4
UniProt Entry Name
VAMP4_HUMAN
NCBI Protein Information
vesicle-associated membrane protein 4

NCBI Summary for VAMP4

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. This protein may play a role in trans-Golgi network-to-endosome transport. [provided by RefSeq, Jul 2008]
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UniProt Comments for VAMP4

Function: Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule.

Subunit structure: Identified in a complex containing STX6, STX12, VAMP4 and VTI1A

By similarity.

Subcellular location: Golgi apparatus › trans-Golgi network membrane; Single-pass type IV membrane protein

Probable. Note: Associated with trans Golgi network (TGN) and newly formed immature secretory granules (ISG). Not found on the mature secretory organelles.

Sequence similarities: Belongs to the synaptobrevin family.Contains 1 v-SNARE coiled-coil homology domain.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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