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nck1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647283

The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NCK1, Polyclonal Antibody
Full Product Name
NCK1, NT (NCK1, NCK, Cytoplasmic protein NCK1, NCK adaptor protein 1, SH2/SH3 adaptor protein NCK-alpha)
Product Gene Name
Product Synonym Names
Anti -NCK1, NT (NCK1, NCK, Cytoplasmic protein NCK1, NCK adaptor protein 1, SH2/SH3 adaptor protein NCK-alpha)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chromosome Location
chromosome: Un
Immunogen
NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-92 amino acids from the N-terminal region of human NCK1.

Product Categories/Family for anti-nck1 antibody

Applications Tested/Suitable for anti-nck1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-nck1 antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50

Western Blot (WB) of anti-nck1 antibody

NCK1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NCK1 antibody detected the NCK1 protein (arrow).
Rabbit Anti-nck1 Polyclonal Antibody – Cat# MBS647283

Western Blot (WB) of anti-nck1 antibody

Western blot analysis of NCK1 (arrow) using rabbit polyclonal NCK1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCK1 gene.
Rabbit Anti-nck1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS647283

Immunohistochemistry (IHC) of anti-nck1 antibody

NCK1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NCK1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-nck1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS647283

Other Notes

Small volumes of anti-nck1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for nck1 purchase

MBS647283 is an antibody targeting nck1. Also known as NCK1, NT (NCK1, NCK, Cytoplasmic protein NCK1, NCK adaptor protein 1, SH2/SH3 adaptor protein NCK-alpha). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for nck1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
nck1 protein
NCBI Official Symbol
nck1
Protein Family
NCBI Protein Information
nck1 protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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