C9orf95 (NMRK1) Antibody – Mouse Polyclonal

Nicotinamide adenine dinucleotide (NAD+) is essential for life in all organisms, both as a coenzyme for oxidoreductases and as a source of ADP-ribosyl groups used in various reactions. Nicotinic acid and nicotinamide, collectively known as niacin, are the vitamin precursors of NAD+. Nicotinamide riboside kinases, such as NRK1, function to synthesize NAD+ through nicotinamide mononucleotide using nicotinamide riboside as the precursor (Bieganowski and Brenner, 2004 [PubMed 15137942]).[supplied by OMIM, Mar 2008]

Function: Catalyzes the phosphorylation of nicotinamide riboside (NR) and nicotinic acid riboside (NaR) to form nicotinamide mononucleotide (NMN) and nicotinic acid mononucleotide (NaMN). The enzyme also phosphorylates the antitumor drugs tiazofurin and 3-deazaguanosine. Ref.1

Catalytic activity: ATP + 1-(beta-D-ribofuranosyl)-nicotinamide = ADP + beta-nicotinamide D-ribonucleotide. Ref.1 Ref.6ATP + beta-D-ribosylnicotinate = ADP + nicotinate beta-D-ribonucleotide. Ref.1 Ref.6

Pathway: Cofactor biosynthesis; NAD(+) biosynthesis.

Subunit structure: Monomer. Ref.6

Sequence similarities: Belongs to the uridine kinase family. NRK subfamily.

Biophysicochemical propertiesKinetic parameters:KM=0.088 mM for nicotinamide riboside (with ATP as cosubstrate) Ref.6 Ref.7KM=0.068 mM for nicotinamide riboside (with GTP as cosubstrate)KM=0.27 mM for tiazofurin (with ATP as cosubstrate)KM=0.051 mM for nicotinic acid riboside (with ATP as cosubstrate)KM=17 mM for uridine (with ATP as cosubstrate)pH dependence:Optimum pH is 6.5-9.