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EPHA10 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6474330

MBS6474330 is an antibody targeting EPHA10. Also known as EPHA10, NT (Ephrin Type-A Receptor 10) (MaxLight 650). Reactive in Human. Host: Rabbit. More details

$1060 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
EPHA10, Polyclonal Antibody
Full Product Name
EPHA10, NT (Ephrin Type-A Receptor 10) (MaxLight 650)
Product Gene Name
anti-EPHA10 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Specificity
Recognizes human EPHA10.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
97.205
OMIM
611123
3D Structure
ModBase 3D Structure for Q5JZY3
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 149-179 in the N-terminal region of human EPHA10, UniProt Accession #Q5JZY3. Species sequence homology: chicken, mouse, rat
Conjugate
MaxLight650
Note
Preservative Free

Product Categories/Family for anti-EPHA10 antibody

Antibodies; Protein Kinases
Applications Tested/Suitable for anti-EPHA10 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-EPHA10 antibody
IHC: 1:10-1:50
FLISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-EPHA10 antibody

Western Blot analysis of lysates from Hela, 293T cell line (from left to right, 20ug/lane), using MBS629889 (1:1000/lane). A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
Rabbit Anti-EPHA10 Polyclonal Antibody – Cat# MBS6474330

Western Blot (WB) of anti-EPHA10 antibody

Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS629889. EPHA10 (arrow) was detected using the purified Pab.
Rabbit Anti-EPHA10 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6474330

Western Blot (WB) of anti-EPHA10 antibody

Western Blot analysis in 293 cell lysates (2ug/lane) usingMBS629889, either nontransfected (Lane 1) or transiently transfected with the EPHA10 gene (Lane 2) (Origene Technologies).(2ug/ml)
Rabbit Anti-EPHA10 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6474330

Immunohistochemistry (IHC) of anti-EPHA10 antibody

Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human testis tissue reacted withMBS629889, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS629889 for immunohistochemistry.
Rabbit Anti-EPHA10 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6474330

Product References and Citations for anti-EPHA10 antibody

1. Aasheim, H.C., Biochim. Biophys. Acta 1723(1-3), 1-7 (2005).

Other Notes

Small volumes of anti-EPHA10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EPHA10. It may not necessarily be applicable to this product.

NCBI GI #
150456469
NCBI GeneID
284656
NCBI Accession #
NP_775912
UniProt Primary Accession #
Q5JZY3
UniProt Related Accession #
Q5JZY3
GenBank Nucleotide #
NP_775912.2
NCBI Official Full Name
ephrin type-A receptor 10 isoform 2
NCBI Official Synonym Full Names
EPH receptor A10
NCBI Official Symbol
EPHA10
UniProt Protein Name
Ephrin type-A receptor 10
Protein Family
Ephrin type-A receptor
UniProt Gene Name
EPHA10
UniProt Entry Name
EPHAA_HUMAN
NCBI Protein Information
ephrin type-A receptor 10

NCBI Summary for EPHA10

Ephrin receptors, the largest subfamily of receptor tyrosine kinases (RTKs), and their ephrin ligands are important mediators of cell-cell communication regulating cell attachment, shape, and mobility in neuronal and epithelial cells (Aasheim et al., 2005 [PubMed 15777695]). See MIM 179610 for additional background on Eph receptors and ephrins.[supplied by OMIM, Mar 2008]
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UniProt Comments for EPHA10

EphA10: Receptor for members of the ephrin-A family. Binds to EFNA3, EFNA4 and EFNA5. Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, tyrosine (receptor); Membrane protein, integral; Protein kinase, TK; EC 2.7.10.1; TK group; Eph family

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; transmembrane-ephrin receptor activity; ephrin receptor activity; ATP binding

Biological Process: axon guidance; peptidyl-tyrosine phosphorylation; ephrin receptor signaling pathway
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Research Articles on EPHA10

1. we defined the novel interaction via expression patterns of EphA10s and EphA10 that promote malignant transformation of breast cancer, and demonstrated the potential benefit in clinical usage.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EPHA10 antibody

 Reference Product  PubMed Publications
 EFNA1 antibody  >1 publications with EPHA10 and EFNA1
 EFNA4 antibody  >1 publications with EPHA10 and EFNA4

Organs/Tissues associated with anti-EPHA10 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >1 publications with EPHA10 and Lung
 Pancreas Antibodies  >1 publications with EPHA10 and Pancreas
 Brain Antibodies  >1 publications with EPHA10 and Brain
 Testis Antibodies  >1 publications with EPHA10 and Testis
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