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EPRS Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6474659

MBS6474659 is an antibody targeting EPRS. Also known as EPRS, NT (Bifunctional aminoacyl-tRNA Synthetase, Cell Proliferation-inducing Gene 32 Protein, Glutamyl-tRNA Synthetase, Glutamate-tRNA Ligase, GluRS, Prolyl-tRNA Synthetase, Proline-tRNA Ligase, GLNS,… More details

$1060 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
EPRS, Polyclonal Antibody
Full Product Name
EPRS, NT (Bifunctional aminoacyl-tRNA Synthetase, Cell Proliferation-inducing Gene 32 Protein, Glutamyl-tRNA Synthetase, Glutamate-tRNA Ligase, GluRS, Prolyl-tRNA Synthetase, Proline-tRNA Ligase, GLNS, PARS, QARS, QPRS, PIG32) (MaxLight 550)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human EPRS.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
170kDa
OMIM
CR933648 mRNA
3D Structure
ModBase 3D Structure for P07814
Immunogen
Synthetic peptide selected from the N-terminal region of human EPRS (KLH).
Conjugate
MaxLight550
Note
Preservative Free

Product Categories/Family for anti-EPRS antibody

Applications Tested/Suitable for anti-EPRS antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-EPRS antibody
IHC: 1:10-1:50
FLISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-EPRS antibody

Western blot analysis of anti-EPRS Pab in Jurkat cell line lysates (35ug/lane). EPRS (arrow) was detected using the purified Pab.
Rabbit Anti-EPRS Polyclonal Antibody – Cat# MBS6474659

Immunohistochemistry (IHC) of anti-EPRS antibody

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EPRS antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-EPRS Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6474659

Product References and Citations for anti-EPRS antibody

1. Jia, J., Mol. Cell 29(6), 679-690 (2008). 2. Beausoleil, S.A., Nat. Biotechnol. 24(10), 1285-1292 (2006). 3. Kato, T., Cancer Res. 65(13), 5638-5646 (2005). 4. Sampath, P., Cell 119(2), 195-208 (2004).

Other Notes

Small volumes of anti-EPRS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EPRS. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
bifunctional glutamate/proline--tRNA ligase
NCBI Official Synonym Full Names
glutamyl-prolyl-tRNA synthetase
NCBI Official Symbol
EPRS
NCBI Official Synonym Symbols
EARS; PARS; QARS; QPRS; HLD15; PIG32; GLUPRORS
UniProt Protein Name
Bifunctional glutamate/proline--tRNA ligase
UniProt Synonym Protein Names
Bifunctional aminoacyl-tRNA synthetase; Cell proliferation-inducing gene 32 protein; Glutamatyl-prolyl-tRNA synthetaseIncluding the following 2 domains:Glutamate--tRNA ligase (EC:6.1.1.17)Alternative name(s):Glutamyl-tRNA synthetase; GluRS
UniProt Gene Name
EPRS
UniProt Synonym Gene Names
GLNS; PARS; QARS; QPRS; GluRS
NCBI Protein Information
bifunctional glutamate/proline--tRNA ligase

NCBI Summary for EPRS

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined. [provided by RefSeq, Jul 2008]
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UniProt Comments for EPRS

Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the multisynthetase complex and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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