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Target of EGR1, Member 1 (TOE1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647488

Inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. Mediates the inhibitory growth effect of EGR1. More details

$850 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Target of EGR1, Member 1 (TOE1), Monoclonal Antibody
Full Product Name
Target of EGR1, Member 1 (Nuclear) (TOE1, FLJ13949)
Product Gene Name
anti-TOE1 antibody
Product Synonym Names
Anti -Target of EGR1, Member 1 (Nuclear) (TOE1, FLJ13949)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8C320
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Target of EGR1, Member 1 (Nuclear).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
56,548 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (45805342..45809650). Location: 1p33
OMIM
613931
3D Structure
ModBase 3D Structure for Q96GM8
Immunogen
Partial sequence of recombinant full-length protein to human Target of EGR1, Member 1 (Nuclear)

Product Categories/Family for anti-TOE1 antibody

Antibodies; Abs to Cell Cycle Inhibitors
Applications Tested/Suitable for anti-TOE1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes for anti-TOE1 antibody
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry.
Dilution: Flow Cytometry: 0.5-2ug/ml
Western Blot: 0.5-2ug/ml
Immunocytochemistry: 2-50ug/ml

Testing Data of anti-TOE1 antibody

Mouse Anti-TOE1 Monoclonal Antibody – Cat# MBS647488

Western Blot (WB) of anti-TOE1 antibody

Detection of human TOE1 by Western blot. Samples: Whole cell lysate (25ug) from HeLa cells. Predicted molecular weight: 56 kD
Mouse Anti-TOE1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS647488

Immunoprecipitation (IP) of anti-TOE1 antibody

Immunoprecipitation: RIPA lysate of HeLa cells was incubated with MBS647488
Mouse Anti-TOE1 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS647488

Testing Data of anti-TOE1 antibody

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with MBS647488 (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG.
Mouse Anti-TOE1 Monoclonal Antibody, Testing Data – Cat# MBS647488

Immunostaining (IS) of anti-TOE1 antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with MBS647488
Mouse Anti-TOE1 Monoclonal Antibody, Immunostaining (IS) – Cat# MBS647488

Other Notes

Small volumes of anti-TOE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TOE1 purchase

MBS647488 is an antibody targeting TOE1. Also known as Target of EGR1, Member 1 (Nuclear) (TOE1, FLJ13949). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TOE1. It may not necessarily be applicable to this product.

NCBI GI #
119627395
NCBI GeneID
114034
NCBI Accession #
EAX06990.1
UniProt Primary Accession #
Q96GM8
UniProt Secondary Accession #
Q6IA35; Q8IWN5; Q9H846
UniProt Related Accession #
Q96GM8
NCBI Official Full Name
target of EGR1, member 1 (nuclear)
NCBI Official Synonym Full Names
target of EGR1, member 1 (nuclear)
NCBI Official Symbol
TOE1
UniProt Protein Name
Target of EGR1 protein 1
Protein Family
Target of EGR1 protein
UniProt Gene Name
TOE1
UniProt Entry Name
TOE1_HUMAN
NCBI Protein Information
target of EGR1 protein 1

UniProt Comments for TOE1

Function: Inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. Mediates the inhibitory growth effect of EGR1. Ref.1

Subcellular location: Nucleus › nucleolus. Nucleus speckle. Note: Localizes to nuclear speckles. Ref.1

Sequence similarities: Belongs to the CAF1 family.Contains 1 C3H1-type zinc finger.
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Research Articles on TOE1

1. TOE1 has a role in the growth inhibitory activity of Egr1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-TOE1 antibody

 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >1 publications with TOE1 and Brain Diseases

Organs/Tissues associated with anti-TOE1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with TOE1 and Brain
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