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ZIC4 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647508

MBS647508 is an antibody targeting ZIC4. Also known as ZIC4 (Zinc Finger Protein of the Cerebellum 4, Zinc Finger Protein ZIC 4). Reactive in Human. Host: Mouse. More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ZIC4, Monoclonal Antibody
Full Product Name
ZIC4 (Zinc Finger Protein of the Cerebellum 4, Zinc Finger Protein ZIC 4)
Product Gene Name
Product Synonym Names
Anti -ZIC4 (Zinc Finger Protein of the Cerebellum 4, Zinc Finger Protein ZIC 4)
Immunogen Sequence
SDRKKHSHVH TSDKPYTCKV RGCDKCYTHP SSLRKHMKVH GRSPPPSSGY DSATPSALVS PSSDCGHKSQ V
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1A9
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human ZIC4.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
36,567 Da
Chromosome Location
Chromosome: 3; NC_000003.11 (147103835..147124596, complement). Location: 3q24
OMIM
608948
3D Structure
ModBase 3D Structure for Q8N9L1
Immunogen
Partial recombinant corresponding to aa249-320 from human ZIC4 (NP_115529) with GST tag. MW of the GST tag alone is 26kD.
Applications Tested/Suitable for anti-ZIC4 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-ZIC4 antibody
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Dilution: Immunofluorescence: 10ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml

Western Blot (WB) of anti-ZIC4 antibody

Western Blot detection against Immunogen (33.92kD).
Mouse Anti-ZIC4 Monoclonal Antibody – Cat# MBS647508

Western Blot (WB) of anti-ZIC4 antibody

ZIC4 monoclonal antibody, Western Blot analysis of ZIC4 expression in Hela NE.
Mouse Anti-ZIC4 Monoclonal Antibody, Western Blot (WB) – Cat# MBS647508

Immunohistochemistry (IHC) of anti-ZIC4 antibody

Immunoperoxidase of monoclonal antibody to ZIC4 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].
Mouse Anti-ZIC4 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS647508

Immunofluorescence (IF) of anti-ZIC4 antibody

Immunofluorescence of monoclonal antibody to ZIC4 on HeLa cell. [antibody concentration 10ug/ml].
Mouse Anti-ZIC4 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS647508

Testing Data of anti-ZIC4 antibody

Detection limit for recombinant GST tagged ZIC4 is ~0.03ng/ml as a capture antibody
Mouse Anti-ZIC4 Monoclonal Antibody, Testing Data – Cat# MBS647508

Other Notes

Small volumes of anti-ZIC4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ZIC4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
zinc finger protein ZIC 4 isoform 4
NCBI Official Synonym Full Names
Zic family member 4
NCBI Official Symbol
ZIC4
UniProt Protein Name
Zinc finger protein ZIC 4
UniProt Synonym Protein Names
Zinc finger protein of the cerebellum 4
Protein Family
UniProt Gene Name
ZIC4
UniProt Entry Name
ZIC4_HUMAN
NCBI Protein Information
zinc finger protein ZIC 4; zinc family member 4 protein HZIC4; zinc finger protein of the cerebellum 4

NCBI Summary for ZIC4

This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development, and have been associated with X-linked visceral heterotaxy and holoprosencephaly type 5. This gene is closely linked to the gene encoding zinc finger protein of the cerebellum 1, a related family member on chromosome 3. Heterozygous deletion of these linked genes is involved in Dandy-Walker malformation, which is a congenital cerebellar malformation. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Dec 2009]
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UniProt Comments for ZIC4

Function: Binds to DNA

By similarity.

Subcellular location: Nucleus

By similarity.

Sequence similarities: Belongs to the GLI C2H2-type zinc-finger protein family.Contains 5 C2H2-type zinc fingers.

Caution: It is uncertain whether Met-1 is the initiator.

Sequence caution: The sequence AAH29507.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence starting in position 229.The sequence AAK06845.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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