Rabbit Anti-PYGB Polyclonal Antibody – Cat# MBS647535

PYGB Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the PYGB antibody detected the PYGB protein (arrow).

PYGB Antibody – Rabbit Polyclonal

Name: PYGB Antibody; Polyclonal; Rabbit; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS647535
Price: 895 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647535

The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PYGB, Polyclonal Antibody

Full Product Name

PYGB, NT (PYGB, Glycogen phosphorylase, brain form)

Product Gene Name

anti-Pygb antibody

Product Synonym Names

Anti -PYGB, NT (PYGB, Glycogen phosphorylase, brain form)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Mouse

Form/Format

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Mouse

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

96,174 Da

Chromosome Location

Chromosome: 3; NC_005102.3 (152941739..152988417). Location: 3q41

3D Structure

ModBase 3D Structure for P53534

Immunogen

PYGB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PYGB.

Product Categories/Family for anti-Pygb antibody

Antibodies; Abs to Enzymes; Phosphorylase

Applications Tested/Suitable for anti-Pygb antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-Pygb antibody

Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Other Notes

Small volumes of anti-Pygb antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Pygb purchase

MBS647535 is an antibody targeting Pygb. Also known as PYGB, NT (PYGB, Glycogen phosphorylase, brain form). Reactive in Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Pygb. It may not necessarily be applicable to this product.

NCBI GI #

183986548

NCBI GeneID

25739

NCBI Accession #

AAI66475.1

UniProt Primary Accession #

P53534

UniProt Related Accession #

P53534

NCBI Official Full Name

Pygb protein

NCBI Official Synonym Full Names

phosphorylase, glycogen; brain

NCBI Official Symbol

Pygb

NCBI Official Synonym Symbols

GLYPHOA

UniProt Protein Name

Glycogen phosphorylase, brain form

Protein Family

Glycogen phosphorylase

UniProt Gene Name

Pygb

UniProt Entry Name

PYGB_RAT

NCBI Protein Information

glycogen phosphorylase, brain form; brain glycogen phosphorylase

NCBI Summary for Pygb

brain specific isozyme of glycogen phosphorylase; poorly activated by phosphorylation [RGD, Feb 2006]

UniProt Comments for Pygb

Function: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.

Catalytic activity: ((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate.

Cofactor: Pyridoxal phosphate.

Enzyme regulation: Activity of phosphorylase is controlled both by allosteric means (through the noncovalent binding of metabolites) and by covalent modification. Thus AMP allosterically activates, whereas ATP, ADP, and glucose-6-phosphate allosterically inhibit, phosphorylase B.

Subunit structure: Homodimer. Dimers associate into a tetramer to form the enzymatically active phosphorylase A.

Post-translational modification: Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.

Sequence similarities: Belongs to the glycogen phosphorylase family.

Research Articles on Pygb

1. Thus, glycogen is an active fuel for specific structures in normal activated brain, not simply an emergency fuel depot and flux-generated pyruvate greatly exceeded net accumulation of lactate or net consumption of glycogen during activation. T

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Pygb antibody

Products by Pathway	Pathway Diagram
Glucose Metabolism Pathway antibodies	Glucose Metabolism Pathway Diagram
Glycogen Metabolism Pathway antibodies	Glycogen Metabolism Pathway Diagram
Glycogen Breakdown (glycogenolysis) Pathway antibodies	Glycogen Breakdown (glycogenolysis) Pathway Diagram
Insulin Signaling Pathway antibodies	Insulin Signaling Pathway Diagram
Insulin Signaling Pathway antibodies	Insulin Signaling Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Carbohydrates Pathway antibodies	Metabolism Of Carbohydrates Pathway Diagram
PKA-HCG-Glycogen Syntase Pathway antibodies	PKA-HCG-Glycogen Syntase Pathway Diagram
Starch And Sucrose Metabolism Pathway antibodies	Starch And Sucrose Metabolism Pathway Diagram
Starch And Sucrose Metabolism Pathway antibodies	Starch And Sucrose Metabolism Pathway Diagram

Diseases associated with anti-Pygb antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>2 publications with Pygb and Neoplasms
Inflammation Antibodies	>2 publications with Pygb and Inflammation
Carcinoma, Hepatocellular Antibodies	>1 publications with Pygb and Carcinoma, Hepatocellular
Disease Models, Animal Antibodies	>1 publications with Pygb and Disease Models, Animal
Adenocarcinoma Antibodies	>1 publications with Pygb and Adenocarcinoma
Brain Diseases Antibodies	>1 publications with Pygb and Brain Diseases
Liver Diseases Antibodies	>1 publications with Pygb and Liver Diseases
Breast Neoplasms Antibodies	>1 publications with Pygb and Breast Neoplasms
Insulin Resistance Antibodies	>1 publications with Pygb and Insulin Resistance
Kidney Diseases Antibodies	>1 publications with Pygb and Kidney Diseases

Organs/Tissues associated with anti-Pygb antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>4 publications with Pygb and Brain
Kidney Antibodies	>1 publications with Pygb and Kidney