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METTL11B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647542

METTL11B is alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di-or tri-methylation of exposed alpha-amino group… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
METTL11B, Polyclonal Antibody
Full Product Name
METTL11B, NT (METTL11B, C1orf184, Alpha N-terminal protein methyltransferase 1B, Methyltransferase-like protein 11B, X-Pro-Lys N-terminal protein methyltransferase 1B)
Product Gene Name
Product Synonym Names
Anti -METTL11B, NT (METTL11B, C1orf184, Alpha N-terminal protein methyltransferase 1B, Methyltransferase-like protein 11B, X-Pro-Lys N-terminal protein methyltransferase 1B)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
32,400 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (170115188..170136923). Location: 1q24.2
3D Structure
ModBase 3D Structure for Q5VVY1
Immunogen
METTL11B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human METTL11B.
Applications Tested/Suitable for anti-METTL11B antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-METTL11B antibody
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Western Blot (WB) of anti-METTL11B antibody

METTL11B Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the METTL11B antibody detected the METTL11B protein (arrow).
Rabbit Anti-METTL11B Polyclonal Antibody – Cat# MBS647542

Other Notes

Small volumes of anti-METTL11B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for METTL11B purchase

MBS647542 is an antibody targeting METTL11B. Also known as METTL11B, NT (METTL11B, C1orf184, Alpha N-terminal protein methyltransferase 1B, Methyltransferase-like protein 11B, X-Pro-Lys N-terminal protein methyltransferase 1B). Reactive in Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for METTL11B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
METTL11B protein
NCBI Official Synonym Full Names
methyltransferase like 11B
NCBI Official Symbol
METTL11B
NCBI Official Synonym Symbols
NTM1B; HOMT1B; C1orf184
UniProt Protein Name
Alpha N-terminal protein methyltransferase 1B
UniProt Synonym Protein Names
Methyltransferase-like protein 11B; X-Pro-Lys N-terminal protein methyltransferase 1B
UniProt Gene Name
METTL11B
UniProt Synonym Gene Names
C1orf184; NTM1B
UniProt Entry Name
NTM1B_HUMAN
NCBI Protein Information
alpha N-terminal protein methyltransferase 1B; methyltransferase-like protein 11B; X-Pro-Lys N-terminal protein methyltransferase 1B

UniProt Comments for METTL11B

Function: Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif

By similarity.

Catalytic activity: 3 S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein].2 S-adenosyl-L-methionine + N-terminal-PPK-[protein] = 2 S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein].

Sequence similarities: Belongs to the methyltransferase superfamily. NTM1 family.

Sequence caution: The sequence CAH72139.1 differs from that shown. Reason: Erroneous gene model prediction.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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