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Fascin (FSCN1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6475668

MBS6475668 is an antibody targeting FSCN1. Also known as Fascin, ID (Singed-like Protein, 55kD Actin-bundling Protein, p55, SNL, HSN, FAN1, FSCN1) (HRP). Reactive in Human. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Fascin (FSCN1), Polyclonal Antibody
Full Product Name
Fascin, ID (Singed-like Protein, 55kD Actin-bundling Protein, p55, SNL, HSN, FAN1, FSCN1) (HRP)
Product Gene Name
anti-FSCN1 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human Fascin.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Molecular Weight
54,530 Da
OMIM
602689
3D Structure
ModBase 3D Structure for Q16658
Immunogen
Synthetic peptide selected from the center region of human Fascin (KLH).
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-FSCN1 antibody

Antibodies; Actin Related Proteins
Applications Tested/Suitable for anti-FSCN1 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-FSCN1 antibody
IHC: 1:50-1:100
ELISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-FSCN1 antibody

Western blot analysis of Fascin Antibody (Center) in Y79 cell line lysates (35ug/lane). Fascin (arrow) was detected using the purified Pab.
Rabbit Anti-FSCN1 Polyclonal Antibody – Cat# MBS6475668

Immunohistochemistry (IHC) of anti-FSCN1 antibody

Formalin-fixed and paraffin-embedded human lung carcinoma with Fascin Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-FSCN1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6475668

Flow Cytometry (FC/FACS) of anti-FSCN1 antibody

Flow cytometric analysis of NCI-H292 cells using Fascin Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-FSCN1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6475668

Product References and Citations for anti-FSCN1 antibody

1. Qualtrough, D., Br. J. Cancer 101(7), 1124-1129 (2009). 2. Ono, S., J. Biol. Chem. 272(4), 2527-2533 (1997).

Other Notes

Small volumes of anti-FSCN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FSCN1. It may not necessarily be applicable to this product.

NCBI GI #
4507115
NCBI GeneID
6624
NCBI Accession #
NP_003079
UniProt Primary Accession #
Q16658
UniProt Secondary Accession #
Q96IC5; Q96IH1; Q9BRF1; A6NI89; B2RE97
UniProt Related Accession #
Q16658
GenBank Nucleotide #
NM_003088.3
NCBI Official Full Name
fascin
NCBI Official Synonym Full Names
fascin actin-bundling protein 1
NCBI Official Symbol
FSCN1
NCBI Official Synonym Symbols
HSN; SNL; p55; FAN1
UniProt Protein Name
Fascin
UniProt Synonym Protein Names
55 kDa actin-bundling protein; Singed-like protein; p55
Protein Family
Fascin
UniProt Gene Name
FSCN1
UniProt Synonym Gene Names
FAN1; HSN; SNL
UniProt Entry Name
FSCN1_HUMAN
NCBI Protein Information
fascin

NCBI Summary for FSCN1

This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011]
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UniProt Comments for FSCN1

Fascin: Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Associates with beta-catenin. Ubiquitous. Belongs to the fascin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Actin-binding

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: cytoplasm; stress fiber; cell junction; cytosol; actin cytoskeleton; filopodium

Molecular Function: protein binding, bridging; actin filament binding; protein binding; drug binding; actin binding

Biological Process: cell proliferation; actin filament bundle formation; cell migration; cell motility involved in cell locomotion; actin cytoskeleton organization and biogenesis
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Research Articles on FSCN1

1. MYC-nick, fascin, and Cdc42 are frequently up-regulated in cells present at the invasive front of human colorectal tumors, suggesting a coordinated role for these proteins in tumor migration.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-FSCN1 antibody

 Products by Pathway  Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Interleukin-4 And 13 Signaling Pathway antibodies  Interleukin-4 And 13 Signaling Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 Signaling By Interleukins Pathway antibodies  Signaling By Interleukins Pathway Diagram

Diseases associated with anti-FSCN1 antibody

 Disease Name  Pubmed Publications
 Neoplasm Metastasis Antibodies  >25 publications with FSCN1 and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >10 publications with FSCN1 and Breast Neoplasms
 Ovarian Neoplasms Antibodies  >7 publications with FSCN1 and Ovarian Neoplasms
 Urinary Bladder Neoplasms Antibodies  >6 publications with FSCN1 and Urinary Bladder Neoplasms
 Lung Neoplasms Antibodies  >6 publications with FSCN1 and Lung Neoplasms
 Liver Neoplasms Antibodies  >5 publications with FSCN1 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >5 publications with FSCN1 and Carcinoma, Hepatocellular
 Lung Diseases Antibodies  >5 publications with FSCN1 and Lung Diseases
 Pancreatic Neoplasms Antibodies  >4 publications with FSCN1 and Pancreatic Neoplasms
 Liver Diseases Antibodies  >4 publications with FSCN1 and Liver Diseases

Organs/Tissues associated with anti-FSCN1 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >13 publications with FSCN1 and Lung
 Lymph Node Antibodies  >13 publications with FSCN1 and Lymph Node
 Blood Antibodies  >10 publications with FSCN1 and Blood
 Stomach Antibodies  >9 publications with FSCN1 and Stomach
 Bladder Antibodies  >8 publications with FSCN1 and Bladder
 Brain Antibodies  >7 publications with FSCN1 and Brain
 Liver Antibodies  >5 publications with FSCN1 and Liver
 Bone Antibodies  >5 publications with FSCN1 and Bone
 Vascular Antibodies  >4 publications with FSCN1 and Vascular
 Muscle Antibodies  >4 publications with FSCN1 and Muscle
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