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FARSA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6475813

MBS6475813 is an antibody targeting FARSA. Also known as FARSA, NT (Phenylalanyl-tRNA Synthetase alpha Chain, Phenylalanine-tRNA Ligase alpha Chain, PheRS, CML33, FARS, FARSL, FARSLA) (HRP). Reactive in Human. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
FARSA, Polyclonal Antibody
Full Product Name
FARSA, NT (Phenylalanyl-tRNA Synthetase alpha Chain, Phenylalanine-tRNA Ligase alpha Chain, PheRS, CML33, FARS, FARSL, FARSLA) (HRP)
Product Gene Name
anti-FARSA antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human FARSA.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Molecular Weight
54,158 Da
OMIM
602918
3D Structure
ModBase 3D Structure for Q9Y285
Immunogen
Synthetic peptide selected from the N-terminal region of human FARSA (KLH).
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-FARSA antibody

Antibodies; DNA/RNA Translation
Applications Tested/Suitable for anti-FARSA antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-FARSA antibody
IHC: 1:10-1:50
ELISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-FARSA antibody

Western blot analysis of anti-FARSA Pab in K562 cell line lysates (35ug/lane).FARSA (arrow) was detected using the purified Pab.
Rabbit Anti-FARSA Polyclonal Antibody – Cat# MBS6475813

Immunohistochemistry (IHC) of anti-FARSA antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with FARSA antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-FARSA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6475813

Product References and Citations for anti-FARSA antibody

1. Gevaert, K., Nat. Biotechnol. 21(5), 566-569 (2003). 2. Moor, N., Protein Expr. Purif. 24(2), 260-267 (2002). 3. Sen, S., Proc. Natl. Acad. Sci. USA 94(12), 6164-6169 (1997).

Other Notes

Small volumes of anti-FARSA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FARSA. It may not necessarily be applicable to this product.

NCBI GI #
4758340
NCBI GeneID
2193
NCBI Accession #
NP_004452
UniProt Primary Accession #
Q9Y285
UniProt Secondary Accession #
Q9NSD8; Q9Y4W8; B4E363
UniProt Related Accession #
Q9Y285
GenBank Nucleotide #
NM_004461
NCBI Official Full Name
phenylalanine--tRNA ligase alpha subunit
NCBI Official Synonym Full Names
phenylalanyl-tRNA synthetase, alpha subunit
NCBI Official Symbol
FARSA
NCBI Official Synonym Symbols
FRSA; CML33; FARSL; PheHA; FARSLA
UniProt Protein Name
Phenylalanine--tRNA ligase alpha subunit
UniProt Synonym Protein Names
CML33; Phenylalanyl-tRNA synthetase alpha subunit; PheRS
Protein Family
Phenylalanine--tRNA ligase
UniProt Gene Name
FARSA
UniProt Synonym Gene Names
FARS; FARSL; FARSLA; PheRS
UniProt Entry Name
SYFA_HUMAN
NCBI Protein Information
phenylalanine--tRNA ligase alpha subunit; pheRS; phenylalanine tRNA ligase 1, alpha, cytoplasmic; phenylalanine--tRNA ligase alpha chain; phenylalanine-tRNA synthetase alpha-subunit; phenylalanine-tRNA synthetase-like, alpha subunit; phenylalanyl-tRNA syn

UniProt Comments for FARSA

FARSLA: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a product which is similar to the catalytic subunit of prokaryotic and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases (PheRS). This gene product has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase gene family shown to exhibit this type of regulated expression [provided by RefSeq, Jul 2008]

Protein type: Translation; EC 6.1.1.20; Ligase; RNA-binding

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: protein binding; phenylalanine-tRNA ligase activity; ATP binding; tRNA binding

Biological Process: tRNA aminoacylation for protein translation; phenylalanyl-tRNA aminoacylation; gene expression
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-FARSA antibody

 Products by Pathway  Pathway Diagram
 Aminoacyl-tRNA Biosynthesis Pathway antibodies  Aminoacyl-tRNA Biosynthesis Pathway Diagram
 Aminoacyl-tRNA Biosynthesis Pathway antibodies  Aminoacyl-tRNA Biosynthesis Pathway Diagram
 Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway antibodies  Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway Diagram
 Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway antibodies  Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway Diagram
 Cytosolic TRNA Aminoacylation Pathway antibodies  Cytosolic TRNA Aminoacylation Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 TRNA Aminoacylation Pathway antibodies  TRNA Aminoacylation Pathway Diagram

Organs/Tissues associated with anti-FARSA antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2 publications with FARSA and Blood
 Skin Antibodies  >2 publications with FARSA and Skin
 Brain Antibodies  >1 publications with FARSA and Brain
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