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POLR3D Antibody – Mouse Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647646

POLR3D is a DNA dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses and acts as nuclear… More details

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Product Overview

Online Datasheet in PDF
Product Name
POLR3D, Polyclonal Antibody
Full Product Name
POLR3D (DNA-directed RNA Polymerase III Subunit RPC4, RNA Polymerase III Subunit C4, DNA-directed RNA Polymerase III Subunit D, Protein BN51, RNA Polymerase III 47 kDa Subunit, RPC53 Homolog, BN51, BN51T)
Product Gene Name
anti-POLR3D antibody
Product Synonym Names
Anti -POLR3D (DNA-directed RNA Polymerase III Subunit RPC4, RNA Polymerase III Subunit C4, DNA-directed RNA Polymerase III Subunit D, Protein BN51, RNA Polymerase III 47 kDa Subunit, RPC53 Homolog, BN51, BN51T)
Immunogen Sequence
MSEGNAAGEP STPGGPRPLL TGARGLIGRR PAPPLTPGRL PSIRSRDLTL GGVKKKTFTP NIISRKIKEE PKEEVTVKKE KRERDRDRQR EGHGRGRGRP EVIQSHSIFE QGPAEMMKKK GNWDKTVDVS DMGPSHIINI KKEKRETDEE TKQILRMLEK DDFLDDPGLR NDTRNMPVQL PLAHSGWLFK EENDEPDVKP WLAGPKEEDM EVDIPAVKVK EEPRDEEEEA KMKAPPKAAR KTPGLPKDVS VAELLRELSL TKEEELLFLQ LPDTLPGQPP TQDIKPIKTE VQGEDGQVVL IKQEKDREAK LAENACTLAD LTEGQVGKLL IRKSGRVQLL LGKVTLDVTM GTACSFLQEL VSVGLGDSRT GEMTVLGHVK HKLVCSPDFE SLLDHKHR
Clonality
Polyclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human POLR3D.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
44,396 Da
Chromosome Location
Chromosome: 8; NC_000008.10 (22102619..22108680). Location: 8q21
OMIM
187280
3D Structure
ModBase 3D Structure for P05423
Immunogen
Full length human POLR3D, aa1-398 (NP_001713.2).

Product Categories/Family for anti-POLR3D antibody

Antibodies; Abs to Enzymes; Polymerase
Applications Tested/Suitable for anti-POLR3D antibody
Western Blot (WB)
Application Notes for anti-POLR3D antibody
Suitable for use in Western Blot.

Western Blot (WB) of anti-POLR3D antibody

POLR3D polyclonal antibody. Western Blot analysis of POLR3D expression in HeLa.
Mouse Anti-POLR3D Polyclonal Antibody – Cat# MBS647646

Western Blot (WB) of anti-POLR3D antibody

POLR3D polyclonal antibody. Western Blot analysis of POLR3D expression in Hela NE.
Mouse Anti-POLR3D Polyclonal Antibody, Western Blot (WB) – Cat# MBS647646

Western Blot (WB) of anti-POLR3D antibody

Western Blot analysis of POLR3D expression in transfected 293T cell line by POLR3D polyclonal antibody. Lane 1: POLR3D transfected lysate (43.78kD). Lane 2: Non-transfected lysate.
Mouse Anti-POLR3D Polyclonal Antibody, Western Blot (WB) – Cat# MBS647646

Other Notes

Small volumes of anti-POLR3D antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POLR3D purchase

MBS647646 is an antibody targeting POLR3D. Also known as POLR3D (DNA-directed RNA Polymerase III Subunit RPC4, RNA Polymerase III Subunit C4, DNA-directed RNA Polymerase III Subunit D, Protein BN51, RNA Polymerase III 47 kDa Subunit, RPC53 Homolog, BN51, BN51T). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for POLR3D. It may not necessarily be applicable to this product.

NCBI GI #
49456563
NCBI GeneID
661
NCBI Accession #
CAG46602.1
UniProt Primary Accession #
P05423
UniProt Secondary Accession #
Q6FI28; Q9BPV7; Q9BPZ1; Q9BXB3
UniProt Related Accession #
P05423
NCBI Official Full Name
POLR3D
NCBI Official Synonym Full Names
polymerase (RNA) III (DNA directed) polypeptide D, 44kDa
NCBI Official Symbol
POLR3D
NCBI Official Synonym Symbols
RPC4; BN51T; RPC53; TSBN51
UniProt Protein Name
DNA-directed RNA polymerase III subunit RPC4
UniProt Synonym Protein Names
DNA-directed RNA polymerase III subunit D; Protein BN51; RNA polymerase III 47 kDa subunit; RPC53 homolog
UniProt Gene Name
POLR3D
UniProt Synonym Gene Names
BN51; BN51T; RNA polymerase III subunit C4
UniProt Entry Name
RPC4_HUMAN
NCBI Protein Information
DNA-directed RNA polymerase III subunit RPC4; RPC53 homolog; RNA polymerase III subunit C4; RNA polymerase III 47 kDa subunit; DNA-directed RNA polymerase III subunit D; BN51 (BHK21) temperature sensitivity complementing; DNA-directed RNA polymerase III 47 kDa polypeptide; temperature sensitive complementation, cell cycle specific, tsBN51

NCBI Summary for POLR3D

This gene complements a temperature-sensitive mutant isolated from the BHK-21 Syrian hamster cell line. It leads to a block in progression through the G1 phase of the cell cycle at nonpermissive temperatures. [provided by RefSeq, Jul 2008]

UniProt Comments for POLR3D

Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway

By similarity. Ref.7 Ref.8

Subunit structure: Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits

By similarity. Interacts with POLR3E/RPC5. Ref.2

Subcellular location: Nucleus.

Sequence similarities: Belongs to the eukaryotic RPC4/POLR3D RNA polymerase subunit family.

Sequence caution: The sequence BC000516 differs from that shown. Reason: Frameshift at position 175. The sequence BC003039 differs from that shown. Reason: Frameshift at position 175.
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Research Articles on POLR3D

1. Studies indicate that aatients with anti-RNAP have an increased risk of malignancy within a 5-year timeframe before or after onset of systemic sclerosis (SSc) skin changes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-POLR3D antibody

 Products by Pathway  Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Eukaryotic Transcription Initiation Pathway antibodies  Eukaryotic Transcription Initiation Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
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