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P4HA1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647688

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
P4HA1, Polyclonal Antibody
Full Product Name
P4HA1, ID (P4HA, Prolyl 4-hydroxylase Subunit alpha-1, 4-PH alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase Subunit alpha-1)
Product Gene Name
anti-P4HA1 antibody
Product Synonym Names
Anti -P4HA1, ID (P4HA, Prolyl 4-hydroxylase Subunit alpha-1, 4-PH alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase Subunit alpha-1)
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human
Form/Format
Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human P4HA1. Species sequence homology: Canine, mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
61,049 Da
Chromosome Location
Chromosome: 10; NC_000010.10 (74766975..74856732, complement). Location: 10q21.3-q23.1
OMIM
176710
3D Structure
ModBase 3D Structure for P13674
Immunogen
Synthetic peptide corresponding to C-DFARKDEPDAFKE of human P4HA1.

Product Categories/Family for anti-P4HA1 antibody

Antibodies; Abs to Enzymes; Oxidase
Applications Tested/Suitable for anti-P4HA1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-P4HA1 antibody
Suitable for use in Western Blot, ELISA and Immunohistochemistry.
Dilution: ELISA: 1:32,000
Western Blot: 1:32,000, 0.1ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml

Immunohistochemistry (IHC) of anti-P4HA1 antibody

Human Skin (formalin-fixed, paraffin-embedded) stained with MBS647688 (5ug/ml) followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.
Goat Anti-P4HA1 Polyclonal Antibody – Cat# MBS647688

Immunohistochemistry (IHC) of anti-P4HA1 antibody

Human Spleen (formalin-fixed, paraffin-embedded) stained with MBS647688 (5ug/ml) followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.
Goat Anti-P4HA1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS647688

Testing Data of anti-P4HA1 antibody

Staining of HEK293 cell lysate (35ug protein in RIPA buffer) using MBS647688 (0.2ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-P4HA1 Polyclonal Antibody, Testing Data – Cat# MBS647688

Other Notes

Small volumes of anti-P4HA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for P4HA1 purchase

MBS647688 is an antibody targeting P4HA1. Also known as P4HA1, ID (P4HA, Prolyl 4-hydroxylase Subunit alpha-1, 4-PH alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase Subunit alpha-1). Reactive in Human. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for P4HA1. It may not necessarily be applicable to this product.

NCBI GI #
217272851
NCBI GeneID
5033
NCBI Accession #
NP_001136068.1
UniProt Primary Accession #
P13674
UniProt Secondary Accession #
Q15082; Q15083; Q5VSQ5; C9JL12
UniProt Related Accession #
P13674
GenBank Nucleotide #
NM_001142596.1
NCBI Official Full Name
prolyl 4-hydroxylase subunit alpha-1 isoform 3
NCBI Official Synonym Full Names
prolyl 4-hydroxylase, alpha polypeptide I
NCBI Official Symbol
P4HA1
NCBI Official Synonym Symbols
P4HA
UniProt Protein Name
Prolyl 4-hydroxylase subunit alpha-1
UniProt Synonym Protein Names
Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1
Protein Family
Prolyl 4-hydroxylase
UniProt Gene Name
P4HA1
UniProt Synonym Gene Names
P4HA; 4-PH alpha-1
UniProt Entry Name
P4HA1_HUMAN
NCBI Protein Information
prolyl 4-hydroxylase subunit alpha-1; C-P4Halpha(I); collagen prolyl 4-hydroxylase alpha(I); procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I

UniProt Comments for P4HA1

Function: Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.

Catalytic activity: L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-proline-[procollagen] + succinate + CO2.

Cofactor: Binds 1 Fe2+ ion per subunit.Ascorbate.

Subunit structure: Heterotetramer of two alpha-1 chains and two beta chains (the beta chain is the multi-functional PDI). Ref.8

Subcellular location: Endoplasmic reticulum lumen.

Sequence similarities: Belongs to the P4HA family.Contains 1 Fe2OG dioxygenase domain.Contains 1 TPR repeat.
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Research Articles on P4HA1

1. Overexpression of miR-122 markedly attenuated the expression of P4HA1 via targeting a binding site located at 3'-UTR of P4HA1 mRNA

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-P4HA1 antibody

 Reference Product  PubMed Publications
 P4HB antibody  >2 publications with P4HA1 and P4HB

Pathways associated with anti-P4HA1 antibody

 Products by Pathway  Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram

Diseases associated with anti-P4HA1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >4 publications with P4HA1 and Neoplasms
 Inflammation Antibodies  >4 publications with P4HA1 and Inflammation
 Anoxia Antibodies  >3 publications with P4HA1 and Anoxia
 Breast Neoplasms Antibodies  >3 publications with P4HA1 and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >3 publications with P4HA1 and Cardiovascular Diseases
 Heart Diseases Antibodies  >2 publications with P4HA1 and Heart Diseases
 Atherosclerosis Antibodies  >2 publications with P4HA1 and Atherosclerosis
 Necrosis Antibodies  >2 publications with P4HA1 and Necrosis
 Fibrosis Antibodies  >1 publications with P4HA1 and Fibrosis
 Weight Loss Antibodies  >1 publications with P4HA1 and Weight Loss

Organs/Tissues associated with anti-P4HA1 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >3 publications with P4HA1 and Liver
 Heart Antibodies  >3 publications with P4HA1 and Heart
 Blood Antibodies  >2 publications with P4HA1 and Blood
 Embryonic Tissue Antibodies  >2 publications with P4HA1 and Embryonic Tissue
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