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ASB9 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647716

Substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes at least two forms of creatine… More details

$880 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ASB9, Monoclonal Antibody
Full Product Name
ASB9 (Ankyrin Repeat and SOCS Box Protein 9, ASB-9, DKFZp564L0862, FLJ20636, MGC4954)
Product Gene Name
anti-ASB9 antibody
Product Synonym Names
Anti -ASB9 (Ankyrin Repeat and SOCS Box Protein 9, ASB-9, DKFZp564L0862, FLJ20636, MGC4954)
Immunogen Sequence
MDGKQGGMDG SKPAGPRDFP GIRLLSNPLM GDAVSDWSPM HEAAIHGHQL SLRNLISQGW AVNIITADHV SPLHEACLGG HLSCVKILLK HGAQVNGVTA DWHTPLFNAC VSGSWDCVNL LLQHGASVQP ESDLASPIHE AARRAYGGNI DHKISHLGTP LYLACENQQR ACVKKLLESG ADVNQGKGQD SPLHAVARTA SEELACLLMD FGADTQAKNA EGKRPVELVP PESPLAQLFL EREGASLPKP KP
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1D8
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human ASB9.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
31,858 Da
Chromosome Location
Chromosome: X; NC_000023.10 (15262109..15288589, complement). Location: Xp22.2
OMIM
300890
3D Structure
ModBase 3D Structure for Q96DX5
Immunogen
Full length recombinant protein corresponding to aa1-253 from human ASB9 (AAH01244) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-ASB9 antibody

Antibodies; Abs to SOCS
Applications Tested/Suitable for anti-ASB9 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-ASB9 antibody
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml

Western Blot (WB) of anti-ASB9 antibody

Western Blot detection against Immunogen (53.83kD).
Mouse Anti-ASB9 Monoclonal Antibody – Cat# MBS647716

Western Blot (WB) of anti-ASB9 antibody

Western Blot analysis of ASB9 expression in transfected 293T cell line by ASB9 monoclonal antibody.|Lane 1: ASB9 transfected lysate (31.9kD).|Lane 2: Non-transfected lysate.
Mouse Anti-ASB9 Monoclonal Antibody, Western Blot (WB) – Cat# MBS647716

Immunohistochemistry (IHC) of anti-ASB9 antibody

Immunoperoxidase of monoclonal antibody to ASB9 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 3ug/ml]
Mouse Anti-ASB9 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS647716

Immunoprecipitation (IP) of anti-ASB9 antibody

Immunoprecipitation of ASB9 transfected lysate using ASB9 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ASB9 rabbit polyclonal antibody.
Mouse Anti-ASB9 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS647716

Testing Data of anti-ASB9 antibody

Detection limit for recombinant GST tagged ASB9 is ~0.1ng/ml as a capture antibody.
Mouse Anti-ASB9 Monoclonal Antibody, Testing Data – Cat# MBS647716

Western Blot (WB) of anti-ASB9 antibody

ASB9 monoclonal antibody, Western Blot analysis of ASB9 expression in HepG2.
Mouse Anti-ASB9 Monoclonal Antibody, Western Blot (WB) – Cat# MBS647716

Other Notes

Small volumes of anti-ASB9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ASB9 purchase

MBS647716 is an antibody targeting ASB9. Also known as ASB9 (Ankyrin Repeat and SOCS Box Protein 9, ASB-9, DKFZp564L0862, FLJ20636, MGC4954). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ASB9. It may not necessarily be applicable to this product.

NCBI GI #
72534772
NCBI GeneID
140462
NCBI Accession #
NP_001026909.1
UniProt Primary Accession #
Q96DX5
UniProt Secondary Accession #
Q9BVF5; Q9NWS5; Q9Y4T3; A8K8A5
UniProt Related Accession #
Q96DX5
GenBank Nucleotide #
NM_001031739.2
NCBI Official Full Name
ankyrin repeat and SOCS box protein 9 isoform 1
NCBI Official Synonym Full Names
ankyrin repeat and SOCS box containing 9
NCBI Official Symbol
ASB9
UniProt Protein Name
Ankyrin repeat and SOCS box protein 9
Protein Family
Ankyrin repeat and SOCS box protein
UniProt Gene Name
ASB9
UniProt Synonym Gene Names
ASB-9
UniProt Entry Name
ASB9_HUMAN
NCBI Protein Information
ankyrin repeat and SOCS box protein 9; ASB-9; ankyrin repeat and SOCS box-containing 9; ankyrin repeat and suppressor of cytokine signaling box protein 9

NCBI Summary for ASB9

This gene encodes a member of the ankyrin repeat and suppressor of cytokine signaling (SOCS) box protein family. Members of this family can interact with the elongin B-C adapter complex via their SOCS box domain and further complex with the cullin and ring box proteins to form E3 ubiquitin ligase complexes. They may function to mediate the substrate-recognition of the E3 ubiquitin ligases. A transcribed pseudogene of this gene has been identified on chromosome 15. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]
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UniProt Comments for ASB9

Function: Substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes at least two forms of creatine kinase, CKB and CKMT1A. Ref.5 Ref.6

Pathway: Protein modification; protein ubiquitination.

Subcellular location: Mitochondrion Ref.5.

Tissue specificity: Predominantly expressed in testis, kidney, and liver. Ref.5

Domain: The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin-protein ligase complexes

By similarity.

Sequence similarities: Contains 6 ANK repeats.Contains 1 SOCS box domain.

Sequence caution: The sequence CAB45706.2 differs from that shown. Reason: Erroneous initiation.
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Research Articles on ASB9

1. His103 and Phe107 in hASB9-2 are essential for binding to CKB.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ASB9 antibody

 Reference Product  PubMed Publications
 CUL5 antibody  >1 publications with ASB9 and CUL5
 CKB antibody  >1 publications with ASB9 and CKB

Pathways associated with anti-ASB9 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram

Diseases associated with anti-ASB9 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1 publications with ASB9 and Neoplasms

Organs/Tissues associated with anti-ASB9 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >2 publications with ASB9 and Brain
 Kidney Antibodies  >2 publications with ASB9 and Kidney
 Lung Antibodies  >1 publications with ASB9 and Lung
 Skin Antibodies  >1 publications with ASB9 and Skin
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