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btrc Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647743

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which… More details

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Product Overview

Online Datasheet in PDF
Product Name
BTRC, Polyclonal Antibody
Full Product Name
BTRC, NT (BTRC, BTRCP, FBW1A, FBXW1A, F-box/WD repeat-containing protein 1A, E3RSIkappaB, Epididymis tissue protein Li 2a, F-box and WD repeats protein beta-TrCP, pIkappaBalpha-E3 receptor subunit)
Product Gene Name
anti-btrc antibody
Product Synonym Names
Anti -BTRC, NT (BTRC, BTRCP, FBW1A, FBXW1A, F-box/WD repeat-containing protein 1A, E3RSIkappaB, Epididymis tissue protein Li 2a, F-box and WD repeats protein beta-TrCP, pIkappaBalpha-E3 receptor subunit)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
59,507 Da
3D Structure
ModBase 3D Structure for Q91854
Immunogen
BTRC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-60 amino acids from the N-terminal region of human BTRC.

Product Categories/Family for anti-btrc antibody

Antibodies; Abs to WD Repeat Proteins
Applications Tested/Suitable for anti-btrc antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-btrc antibody
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunofluorescence: 1:10-50

Western Blot (WB) of anti-btrc antibody

BTRC Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the BTRC antibody detected the BTRC protein (arrow).
Rabbit Anti-btrc Polyclonal Antibody – Cat# MBS647743

Immunohistochemistry (IHC) of anti-btrc antibody

BTRC Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BTRC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-btrc Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS647743

Immunofluorescence (IF) of anti-btrc antibody

Confocal immunofluorescent analysis of BTRC Antibody (N-term) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-btrc Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS647743

Other Notes

Small volumes of anti-btrc antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for btrc purchase

MBS647743 is an antibody targeting btrc. Also known as BTRC, NT (BTRC, BTRCP, FBW1A, FBXW1A, F-box/WD repeat-containing protein 1A, E3RSIkappaB, Epididymis tissue protein Li 2a, F-box and WD repeats protein beta-TrCP, pIkappaBalpha-E3 receptor subunit). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for btrc. It may not necessarily be applicable to this product.

NCBI GI #
148236261
NCBI GeneID
394362
NCBI Accession #
NP_001081064.1
UniProt Primary Accession #
Q91854
UniProt Secondary Accession #
P70037; P70038; Q6AX69
UniProt Related Accession #
Q91854
GenBank Nucleotide #
NM_001087595.1
NCBI Official Full Name
beta-TrCP
NCBI Official Synonym Full Names
beta-transducin repeat containing E3 ubiquitin protein ligase
NCBI Official Symbol
btrc
NCBI Official Synonym Symbols
fwd1; btrcp; fbw1a; fbxw1; btrc-a; btrcp1; fbxw1a; betaTrCP; beta-TrCP
UniProt Protein Name
Beta-TrCP
UniProt Synonym Protein Names
Beta-transducin repeat-containing protein
Protein Family
F-box/WD repeat-containing protein
UniProt Gene Name
fbxw1
UniProt Synonym Gene Names
btrcp
UniProt Entry Name
TRCB_XENLA
NCBI Protein Information
beta-TrCP; beta-transducin repeat-containing protein

UniProt Comments for btrc

Function: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. May participate in Wnt signaling.

Subunit structure: Part of a SCF (SKP1-cullin-F-box) ubiquitin-protein ligase complex

By similarity.

Developmental stage: Present in fully grown and progesterone-matured oocytes. The level change very little even after zygotic gene transcription begins following the midblastula transition. Do not increase in abundance in the gastrula, neurula, tailbud, or tadpole embryo.

Sequence similarities: Contains 1 F-box domain.Contains 7 WD repeats.
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Research Articles on btrc

1. Strong ERK activation can target Cdc25A for degradation in a manner similar to, but independent of, Chk1 for cell cycle arrest.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-btrc antibody

 Products by Pathway  Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Oocyte Meiosis Pathway antibodies  Oocyte Meiosis Pathway Diagram
 Oocyte Meiosis Pathway antibodies  Oocyte Meiosis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Wnt Signaling Pathway antibodies  Wnt Signaling Pathway Diagram
 Wnt Signaling Pathway antibodies  Wnt Signaling Pathway Diagram

Diseases associated with anti-btrc antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >27 publications with btrc and Neoplasms
 Necrosis Antibodies  >9 publications with btrc and Necrosis
 Nervous System Diseases Antibodies  >6 publications with btrc and Nervous System Diseases
 Central Nervous System Diseases Antibodies  >5 publications with btrc and Central Nervous System Diseases
 Congenital Abnormalities Antibodies  >5 publications with btrc and Congenital Abnormalities
 Brain Diseases Antibodies  >5 publications with btrc and Brain Diseases
 Inflammation Antibodies  >3 publications with btrc and Inflammation
 Lung Neoplasms Antibodies  >2 publications with btrc and Lung Neoplasms
 Genital Diseases, Male Antibodies  >2 publications with btrc and Genital Diseases, Male
 Cardiovascular Diseases Antibodies  >2 publications with btrc and Cardiovascular Diseases
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