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UBE2J1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647785

MBS647785 is an antibody targeting UBE2J1. Also known as UBE2J1 (Ubiquitin-conjugating Enzyme E2 J1, CGI-76, HSPC153, HSPC205, Non-canonical Ubiquitin-conjugating Enzyme 1, NCUBE1, NCUBE-1, Yeast Ubiquitin-conjugating Enzyme UBC6 Homolog E, HsUBC6e). Reactive… More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
UBE2J1, Monoclonal Antibody
Full Product Name
UBE2J1 (Ubiquitin-conjugating Enzyme E2 J1, CGI-76, HSPC153, HSPC205, Non-canonical Ubiquitin-conjugating Enzyme 1, NCUBE1, NCUBE-1, Yeast Ubiquitin-conjugating Enzyme UBC6 Homolog E, HsUBC6e)
Product Gene Name
Product Synonym Names
Anti -UBE2J1 (Ubiquitin-conjugating Enzyme E2 J1, CGI-76, HSPC153, HSPC205, Non-canonical Ubiquitin-conjugating Enzyme 1, NCUBE1, NCUBE-1, Yeast Ubiquitin-conjugating Enzyme UBC6 Homolog E, HsUBC6e)
Immunogen Sequence
SPAVKRLMKE AAELKDPTDH YHAQPLEDNL FEWHFTVRGP PDSDFDGGVY HGRIVLPPEY PMKPPSIILL TANGRFEVGK KICLSISGHH PETWQPSWSI RTALLAIIGF
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
6A12
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human UBE2J1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
35,199 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (90036344..90062619, complement). Location: 6q15
3D Structure
ModBase 3D Structure for Q9Y385
Immunogen
Partial recombinant corresponding to aa9-119 from human UBE2J1 with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-UBE2J1 antibody

Applications Tested/Suitable for anti-UBE2J1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-UBE2J1 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.1ng/ml

Western Blot (WB) of anti-UBE2J1 antibody

Western Blot detection against Immunogen (38.21kD).
Mouse Anti-UBE2J1 Monoclonal Antibody – Cat# MBS647785

Western Blot (WB) of anti-UBE2J1 antibody

UBE2J1 monoclonal antibody, Western Blot analysis of UBE2J1 expression in HeLa.
Mouse Anti-UBE2J1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS647785

Testing Data of anti-UBE2J1 antibody

Detection limit for recombinant GST tagged UBE2J1 is ~0.1ng/ml as a capture antibody.
Mouse Anti-UBE2J1 Monoclonal Antibody, Testing Data – Cat# MBS647785

Other Notes

Small volumes of anti-UBE2J1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for UBE2J1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ubiquitin-conjugating enzyme E2 J1
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2, J1
NCBI Official Symbol
UBE2J1
NCBI Official Synonym Symbols
UBC6; Ubc6p; CGI-76; NCUBE1; HSPC153; HSPC205; NCUBE-1; HSU93243
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 J1
UniProt Synonym Protein Names
Non-canonical ubiquitin-conjugating enzyme 1; NCUBE-1; Yeast ubiquitin-conjugating enzyme UBC6 homolog E
UniProt Gene Name
UBE2J1
UniProt Synonym Gene Names
NCUBE1; NCUBE-1; HsUBC6e
UniProt Entry Name
UB2J1_HUMAN
NCBI Protein Information
ubiquitin-conjugating enzyme E2 J1; HSUBC6e; ubiquitin-conjugating enzyme E2, J1, U; non-canonical ubiquitin-conjugating enzyme 1; yeast ubiquitin-conjugating enzyme UBC6 homolog E; ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast)

NCBI Summary for UBE2J1

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system. [provided by RefSeq, Jul 2008]
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UniProt Comments for UBE2J1

Function: Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD). Ref.2 Ref.15

Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway: Protein modification; protein ubiquitination.

Subcellular location: Endoplasmic reticulum membrane; Single-pass type IV membrane protein Ref.2.

Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.

Sequence caution: The sequence AAD34071.1 differs from that shown. Reason: Erroneous initiation. The sequence AAF36125.1 differs from that shown. Reason: Frameshift at position 119.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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