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SGPP1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647795

SPP1 binds tightly to hydroxyapatite. The protein appears to form an integral part of the mineralized matrix and probably important to cell-matrix interaction. It acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SPP1 (SGPP1), Polyclonal Antibody
Full Product Name
SPP1, CT (Osteopontin, Bone Sialoprotein 1, Secreted Phosphoprotein 1, SPP-1, Urinary Stone Protein, Nephropontin, Uropontin, OPN, BNSP, PSEC0156)
Product Gene Name
Product Synonym Names
Anti -SPP1, CT (Osteopontin, Bone Sialoprotein 1, Secreted Phosphoprotein 1, SPP-1, Urinary Stone Protein, Nephropontin, Uropontin, OPN, BNSP, PSEC0156)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human SPP1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
49,108 Da
Chromosome Location
Chromosome: 14; NC_000014.8 (64150934..64194756, complement). Location: 14q23.2
OMIM
612826
3D Structure
ModBase 3D Structure for Q9BX95
Immunogen
Synthetic peptide selected from the C-terminal region of human SPP1 (KLH).
Applications Tested/Suitable for anti-SGPP1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-SGPP1 antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry (paraffin): 1:50-1:100

Western Blot (WB) of anti-SGPP1 antibody

Western blot analysis of SPP1 Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). SPP1 (arrow) was detected using the purified Pab.
Rabbit Anti-SGPP1 Polyclonal Antibody – Cat# MBS647795

Immunohistochemistry (IHC) of anti-SGPP1 antibody

Formalin-fixed and paraffin-embedded human lung carcinoma with SPP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-SGPP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS647795

Flow Cytometry (FC/FACS) of anti-SGPP1 antibody

Flow cytometric analysis of NCI-H292 cells using SPP1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-SGPP1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS647795

Other Notes

Small volumes of anti-SGPP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SGPP1 purchase

MBS647795 is an antibody targeting SGPP1. Also known as SPP1, CT (Osteopontin, Bone Sialoprotein 1, Secreted Phosphoprotein 1, SPP-1, Urinary Stone Protein, Nephropontin, Uropontin, OPN, BNSP, PSEC0156). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SGPP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
sphingosine-1-phosphate phosphatase 1
NCBI Official Synonym Full Names
sphingosine-1-phosphate phosphatase 1
NCBI Official Symbol
SGPP1
NCBI Official Synonym Symbols
SPPase1
UniProt Protein Name
Sphingosine-1-phosphate phosphatase 1
UniProt Synonym Protein Names
Sphingosine-1-phosphatase 1
UniProt Gene Name
SGPP1
UniProt Synonym Gene Names
SPPase1; Spp1; hSPP1; hSPPase1
UniProt Entry Name
SGPP1_HUMAN
NCBI Protein Information
sphingosine-1-phosphate phosphatase 1; spp1; hSPP1; hSPPase1; sphingosine-1-phosphatase 1

NCBI Summary for SGPP1

Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP1 catalyzes the degradation of S1P via salvage and recycling of sphingosine into long-chain ceramides (Mandala et al., 2000 [PubMed 10859351]; Le Stunff et al., 2007 [PubMed 17895250]).[supplied by OMIM, Jun 2009]
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UniProt Comments for SGPP1

Function: Has enzymatic activity against both sphingosine 1-phosphate (S1P) and dihydro-S1P. Regulates intracellular and extracellular S1P levels. Ref.1

Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.1.

Tissue specificity: Ubiquitous, with the strongest level in placenta and kidney. Ref.1

Sequence similarities: Belongs to the type 2 lipid phosphate phosphatase family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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