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GCNT1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6477971

MBS6477971 is an antibody targeting GCNT1. Also known as GCNT1, CT (Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase, Core 2 Branching Enzyme, Core2-GlcNAc-transferase, C2GNT, Core 2 GNT, NACGT2) (Azide free) (HRP).… More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GCNT1, Polyclonal Antibody
Full Product Name
GCNT1, CT (Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase, Core 2 Branching Enzyme, Core2-GlcNAc-transferase, C2GNT, Core 2 GNT, NACGT2) (Azide free) (HRP)
Product Gene Name
anti-GCNT1 antibody
Product Synonym Gene Name
G6NT
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Specificity
Recognizes human GCNT1.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
49,799 Da
OMIM
600391
3D Structure
ModBase 3D Structure for Q02742
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 399-428 in the C-terminal region of human GCNT1, UniProt Accession #NP_001481; Q02742.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-GCNT1 antibody

Antibodies; Enzymes; Glycosyltransferase
Applications Tested/Suitable for anti-GCNT1 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-GCNT1 antibody
IHC: 1:50-1:100
ELISA: 1:1000
WB: 1:100-1:500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-GCNT1 antibody

Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS629278. GCNT1 (arrow) was detected using the purified Pab.
Rabbit Anti-GCNT1 Polyclonal Antibody – Cat# MBS6477971

Immunohistochemistry (IHC) of anti-GCNT1 antibody

Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS629278, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS629278 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-GCNT1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6477971

Product References and Citations for anti-GCNT1 antibody

1. Bierhuizen, M.F., et al., Glycobiology 5(4):417-425 (1995). 2. Bierhuizen, M.F., et al., Proc. Natl. Acad. Sci. USA 89(19):9326-9330 (1992).

Other Notes

Small volumes of anti-GCNT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GCNT1. It may not necessarily be applicable to this product.

NCBI GI #
21614523
NCBI GeneID
2650
NCBI Accession #
NP_001481
UniProt Primary Accession #
Q02742
UniProt Secondary Accession #
Q6DJZ4
UniProt Related Accession #
Q02742
GenBank Nucleotide #
NM_001490.4
NCBI Official Full Name
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
NCBI Official Synonym Full Names
glucosaminyl (N-acetyl) transferase 1, core 2
NCBI Official Symbol
GCNT1
NCBI Official Synonym Symbols
G6NT; C2GNT; C2GNT1; NACGT2; NAGCT2; C2GNT-L
UniProt Protein Name
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
UniProt Synonym Protein Names
Core 2-branching enzyme; Core2-GlcNAc-transferase; C2GNT; Core 2 GNT
UniProt Gene Name
GCNT1
UniProt Synonym Gene Names
NACGT2; C2GNT; Core 2 GNT
UniProt Entry Name
GCNT1_HUMAN
NCBI Protein Information
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

NCBI Summary for GCNT1

This gene is a member of the beta-1,6-N-acetylglucosaminyltransferase gene family. It is essential to the formation of Gal beta 1-3(GlcNAc beta 1-6)GalNAc structures and the core 2 O-glycan branch. The gene coding this enzyme was originally mapped to 9q21, but was later localized to 9q13. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
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Research Articles on GCNT1

1. GCNT1 expression in prostate biopsy specimen is a significant and independent predictor of recurrence after radical prostatectomy, which can be used in pre-treatment decision making for the patient.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-GCNT1 antibody

 Products by Pathway  Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Mucin Type O-Glycan Biosynthesis Pathway antibodies  Mucin Type O-Glycan Biosynthesis Pathway Diagram
 Mucin Type O-Glycan Biosynthesis Pathway antibodies  Mucin Type O-Glycan Biosynthesis Pathway Diagram
 O-glycan Biosynthesis, Mucin Type Core Pathway antibodies  O-glycan Biosynthesis, Mucin Type Core Pathway Diagram
 O-glycan Biosynthesis, Mucin Type Core Pathway antibodies  O-glycan Biosynthesis, Mucin Type Core Pathway Diagram
 O-linked Glycosylation Pathway antibodies  O-linked Glycosylation Pathway Diagram
 O-linked Glycosylation Of Mucins Pathway antibodies  O-linked Glycosylation Of Mucins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Mucin Core 1 And Core 2 O-glycosylation Pathway antibodies  Mucin Core 1 And Core 2 O-glycosylation Pathway Diagram

Diseases associated with anti-GCNT1 antibody

 Disease Name  Pubmed Publications
 Prostatic Neoplasms Antibodies  >4 publications with GCNT1 and Prostatic Neoplasms
 Neoplasms, Experimental Antibodies  >1 publications with GCNT1 and Neoplasms, Experimental
 Disease Models, Animal Antibodies  >1 publications with GCNT1 and Disease Models, Animal
 Breast Neoplasms Antibodies  >1 publications with GCNT1 and Breast Neoplasms
 Inflammation Antibodies  >1 publications with GCNT1 and Inflammation

Organs/Tissues associated with anti-GCNT1 antibody

 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >7 publications with GCNT1 and Prostate
 Blood Antibodies  >3 publications with GCNT1 and Blood
 Skin Antibodies  >1 publications with GCNT1 and Skin
 Ovary Antibodies  >1 publications with GCNT1 and Ovary
 Muscle Antibodies  >1 publications with GCNT1 and Muscle
 Lung Antibodies  >1 publications with GCNT1 and Lung
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