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Glutamate Decarboxylase 65,67 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6478175

MBS6478175 is an antibody targeting Glutamate Decarboxylase 65,67. Also known as Glutamate Decarboxylase 65,67 (GAD 65,67) (PE). Reactive in Rat, Feline, Human, Mouse. Host: Rabbit. More details

$1745 / 0.1 mL +2 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Glutamate Decarboxylase 65,67, Polyclonal Antibody
Full Product Name
Glutamate Decarboxylase 65,67 (GAD 65,67) (PE)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Rat, Feline, Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography
Specificity
Recognizes both molecular forms of rat glutamate decarboxylase (GAD65 and GAD67), the enzyme which converts glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in the higher brain region. Species Crossreactivity: human, mouse and feline
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Synthetic peptide corresponding to DFLIEEIERLGQDL from the C-terminus of rat GAD2.
Conjugate
PE
Note
Preservative Free
Applications Tested/Suitable for anti-Glutamate Decarboxylase 65,67 antibody
Immunohistochemistry (IHC) Frozen/Paraffin, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF)
IHC-F: 1:200-1:5000
WB: 1:1000-1:10,000. A doublet at ~65/67kD is expected.
Applications are based on unconjugated antibody.

Product References and Citations for anti-Glutamate Decarboxylase 65,67 antibody

1. Hughes, S.M., et al., Dev. Neurobiol. 69: 477-490 (2009). General References: 1. Hernandez-Montiel, H.L., et al., Dev. Biol. 255: 99-112 (2003). 2. Baizer, J.S., et al., Anat. Rec. (Hoboken) 294: 1198-1216 (2011). 3. Klusa, V., et al., Pharmacol. Biochem. Behav. pii: S0091-3057(13)00078-6 (2013). 4. Wang, X., et al., BMC Biotechnol. 8: 87 (2008).

Other Notes

Small volumes of anti-Glutamate Decarboxylase 65,67 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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