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LGMN Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647828

This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LGMN, Polyclonal Antibody
Full Product Name
LGMN, NT (LGMN, PRSC1, Legumain, Asparaginyl endopeptidase, Protease, cysteine 1)
Product Gene Name
anti-LGMN antibody
Product Synonym Names
Anti -LGMN, NT (LGMN, PRSC1, Legumain, Asparaginyl endopeptidase, Protease, cysteine 1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
49,411 Da
Chromosome Location
Chromosome: 14; NC_000014.8 (93170152..93215047, complement). Location: 14q32.1
OMIM
602620
3D Structure
ModBase 3D Structure for Q99538
Immunogen
LGMN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83-112 amino acids from the N-terminal region of human LGMN.

Product Categories/Family for anti-LGMN antibody

Antibodies; Abs to Enzymes; Protease (Proteinase; Peptidase)
Applications Tested/Suitable for anti-LGMN antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-LGMN antibody
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Western Blot (WB) of anti-LGMN antibody

Western Blot analysis in 293 cell line lysates (35ug/lane) using MBS647828. This demonstrates that MBS647828 detected the LGMN protein (arrow).
Rabbit Anti-LGMN Polyclonal Antibody – Cat# MBS647828

Western Blot (WB) of anti-LGMN antibody

Western Blot analysis in human kidney lysate Lysates (20ug/lane) using MBS647828(1:1000). Secondary IgG (HRP) Goat Anti-Rabbit IgG, 1:10,000). Predicted band size: 49kD. Blocking/Dilution buffer: 5% NFDM/TBST. This demonstrates that MBS647828 detected the LGMN protein (arrow).
Rabbit Anti-LGMN Polyclonal Antibody, Western Blot (WB) – Cat# MBS647828

Other Notes

Small volumes of anti-LGMN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LGMN purchase

MBS647828 is an antibody targeting LGMN. Also known as LGMN, NT (LGMN, PRSC1, Legumain, Asparaginyl endopeptidase, Protease, cysteine 1). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LGMN. It may not necessarily be applicable to this product.

NCBI GI #
56682964
NCBI GeneID
5641
NCBI Accession #
NP_001008530.1
UniProt Primary Accession #
Q99538
UniProt Secondary Accession #
O00123; Q9BTY1
UniProt Related Accession #
Q99538
GenBank Nucleotide #
NM_001008530.2
NCBI Official Full Name
legumain preproprotein
NCBI Official Synonym Full Names
legumain
NCBI Official Symbol
LGMN
NCBI Official Synonym Symbols
AEP; LGMN1; PRSC1
UniProt Protein Name
Legumain
UniProt Synonym Protein Names
Asparaginyl endopeptidase; Protease, cysteine 1
UniProt Gene Name
LGMN
UniProt Synonym Gene Names
PRSC1
UniProt Entry Name
LGMN_HUMAN
NCBI Protein Information
legumain; cysteine protease 1; protease, cysteine 1; asparaginyl endopeptidase; protease, cysteine, 1 (legumain)

UniProt Comments for LGMN

Function: Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.

Catalytic activity: Hydrolysis of proteins and small molecule substrates at -Asn-|-Xaa- bonds.

Subcellular location: Lysosome

By similarity.

Tissue specificity: Ubiquitous. Particularly abundant in kidney, heart and placenta.

Post-translational modification: Glycosylated.

Sequence similarities: Belongs to the peptidase C13 family.
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Research Articles on LGMN

1. The multibranched and context-dependent activation process of legumain illustrates how proteases can act not only as signal transducers but as decision makers.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LGMN antibody

 Reference Product  PubMed Publications
 CTSS antibody  >1 publications with LGMN and CTSS

Pathways associated with anti-LGMN antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 MHC Class II Antigen Presentation Pathway antibodies  MHC Class II Antigen Presentation Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram

Diseases associated with anti-LGMN antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2 publications with LGMN and Neoplasms
 Liver Neoplasms Antibodies  >1 publications with LGMN and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >1 publications with LGMN and Carcinoma, Hepatocellular
 Liver Diseases Antibodies  >1 publications with LGMN and Liver Diseases

Organs/Tissues associated with anti-LGMN antibody

 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >2 publications with LGMN and Embryonic Tissue
 Blood Antibodies  >2 publications with LGMN and Blood
 Pancreas Antibodies  >1 publications with LGMN and Pancreas
 Liver Antibodies  >1 publications with LGMN and Liver
 Uterus Antibodies  >1 publications with LGMN and Uterus
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