Rabbit Anti-HAND1 Polyclonal Antibody – Cat# MBS647876

HAND1 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the HAND1 antibody detected the HAND1 protein (arrow).

HAND1 Antibody – Rabbit Polyclonal

Name: HAND1 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS647876
Price: 895 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647876

The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular… More details

$895 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

HAND1, Polyclonal Antibody

Full Product Name

HAND1, ID (HAND1, BHLHA27, EHAND, Heart- and neural crest derivatives-expressed protein 1, Class A basic helix-loop-helix protein 27, Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1)

Product Gene Name

anti-HAND1 antibody

Product Synonym Names

Anti -HAND1, ID (HAND1, BHLHA27, EHAND, Heart- and neural crest derivatives-expressed protein 1, Class A basic helix-loop-helix protein 27, Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Human

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

23,627 Da

Chromosome Location

Chromosome: 5; NC_000005.9 (153854532..153857824, complement). Location: 5q33

OMIM

602406

3D Structure

ModBase 3D Structure for O96004

Immunogen

HAND1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-105 amino acids from the Central region of human HAND1.

Product Categories/Family for anti-HAND1 antibody

Antibodies; Abs to Transcription Factors

Applications Tested/Suitable for anti-HAND1 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-HAND1 antibody

Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Other Notes

Small volumes of anti-HAND1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HAND1 purchase

MBS647876 is an antibody targeting HAND1. Also known as HAND1, ID (HAND1, BHLHA27, EHAND, Heart- and neural crest derivatives-expressed protein 1, Class A basic helix-loop-helix protein 27, Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HAND1. It may not necessarily be applicable to this product.

NCBI GI #

4758506

NCBI GeneID

9421

NCBI Accession #

NP_004812.1

UniProt Primary Accession #

O96004

UniProt Related Accession #

O96004

GenBank Nucleotide #

NM_004821.2

NCBI Official Full Name

heart- and neural crest derivatives-expressed protein 1

NCBI Official Synonym Full Names

heart and neural crest derivatives expressed 1

NCBI Official Symbol

HAND1

NCBI Official Synonym Symbols

Hxt; eHand; Thing1; bHLHa27

UniProt Protein Name

Heart- and neural crest derivatives-expressed protein 1

UniProt Synonym Protein Names

Class A basic helix-loop-helix protein 27; bHLHa27; Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1

Protein Family

Heart- and neural crest derivatives-expressed protein

UniProt Gene Name

HAND1

UniProt Synonym Gene Names

BHLHA27; EHAND; bHLHa27; eHAND

UniProt Entry Name

HAND1_HUMAN

NCBI Protein Information

heart- and neural crest derivatives-expressed protein 1; class A basic helix-loop-helix protein 27; extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1

UniProt Comments for HAND1

Function: Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box)

By similarity.

Subunit structure: Efficient DNA binding requires dimerization with another bHLH protein. Forms homodimers and heterodimers with TCF3 gene products E12 and E47, HAND2 and HEY1, HEY2 and HEYL (hairy-related transcription factors). Interacts with MDFIC

By similarity.

Subcellular location: Nucleus › nucleoplasm

By similarity. Nucleus › nucleolus

By similarity. Note: Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity. Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity

By similarity.

Tissue specificity: Heart.

Post-translational modification: Phosphorylation by PLK4 disrupts the interaction with MDFIC and leads to tranlocation into the nucleoplasm, allowing dimerization and transcription factor activity

By similarity.

Sequence similarities: Contains 1 bHLH (basic helix-loop-helix) domain.

Research Articles on HAND1

1. This is the first report of mutations in the HAND1 gene in Chinese patients with VSD and provides new insight into the etiology of VSD

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HAND1 antibody

Reference Product	PubMed Publications
HAND2 antibody	>54 publications with HAND1 and HAND2
TCF3 antibody	>10 publications with HAND1 and TCF3
TCF12 antibody	>2 publications with HAND1 and TCF12
MYOD1 antibody	>1 publications with HAND1 and MYOD1
FHL2 antibody	>1 publications with HAND1 and FHL2

Pathways associated with anti-HAND1 antibody

Products by Pathway	Pathway Diagram
Heart Development Pathway antibodies	Heart Development Pathway Diagram
Neural Crest Differentiation Pathway antibodies	Neural Crest Differentiation Pathway Diagram

Diseases associated with anti-HAND1 antibody

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>6 publications with HAND1 and Disease Models, Animal
Abnormalities, Multiple Antibodies	>5 publications with HAND1 and Abnormalities, Multiple
Cleft Palate Antibodies	>2 publications with HAND1 and Cleft Palate
Limb Deformities, Congenital Antibodies	>2 publications with HAND1 and Limb Deformities, Congenital
Necrosis Antibodies	>2 publications with HAND1 and Necrosis
Central Nervous System Diseases Antibodies	>1 publications with HAND1 and Central Nervous System Diseases
Bone Marrow Diseases Antibodies	>1 publications with HAND1 and Bone Marrow Diseases
Brain Diseases Antibodies	>1 publications with HAND1 and Brain Diseases

Organs/Tissues associated with anti-HAND1 antibody

Organ/Tissue Name	Pubmed Publications
Embryonic Tissue Antibodies	>61 publications with HAND1 and Embryonic Tissue
Nerve Antibodies	>9 publications with HAND1 and Nerve
Brain Antibodies	>4 publications with HAND1 and Brain
Connective Tissue Antibodies	>1 publications with HAND1 and Connective Tissue
Eye Antibodies	>1 publications with HAND1 and Eye