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NEK11 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647920

Protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation. More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NEK11, Monoclonal Antibody
Full Product Name
NEK11 (Serine/Threonine-protein Kinase Nek11, Never in Mitosis A-related Kinase 11, NimA-related Protein Kinase 11, FLJ23495)
Product Synonym Names
Anti -NEK11 (Serine/Threonine-protein Kinase Nek11, Never in Mitosis A-related Kinase 11, NimA-related Protein Kinase 11, FLJ23495)
Immunogen Sequence
MLKFQEAAKC VSGSTAISTY PKTLIARRYV LQQKLGSGSF GTVYLVSDKK AKRGEELKVL KEISVGELNP NETVQANLEA QLLSKLDHPA IVKFHASFVE QDNFCIITEY CEGRDLDDKI QEYKQAGKIF PENQIIEWFI QLLLGVDYMH ERRILHRDLK SKNVFLKNNL LKIGDFGVSR LLMGSCDLAT TLTGTPHYMS PEALKHQGYD TKSDIWSLAC ILYEMCCMNH AFAGSNFLSI VLKIVEGDTP SLPERYPKEL NAIMESMLNK NPSLRPSAIE ILKIPYLDEQ LQNLMCRYSE MTLEDKNLDC QKEAAHIINA MQKRIHLQTL RALSEVQKMT PRERMRLRKL QAADEKARKL KKIVEEKYEE NSKRMQELRS RNFQQLSVDV LHEKTHLKGM EEKEEQPEGR LSCSPQDEDE ERWQGREEES DEPTLENLPE SQPIPSMDLH ELESIVEDAT SDLGYHGDCN LISLDEYWKN EK
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4E1-1F4
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human NEK11.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full length recombinant corresponding to aa1-483 from human NEK11 (AAH28587) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-NEK11 antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-NEK11 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-NEK11 antibody
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Dilution: Immunofluorescence: 10ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml

Western Blot (WB) of anti-NEK11 antibody

Western Blot detection against Immunogen (79.13kD).
Mouse Anti-NEK11 Monoclonal Antibody – Cat# MBS647920

Western Blot (WB) of anti-NEK11 antibody

Western Blot analysis of NEK11 expression in transfected 293T cell line by NEK11 monoclonal antibody.|Lane 1: NEK11 transfected lysate (55.5kD).|Lane 2: Non-transfected lysate.
Mouse Anti-NEK11 Monoclonal Antibody, Western Blot (WB) – Cat# MBS647920

Immunohistochemistry (IHC) of anti-NEK11 antibody

Immunoperoxidase of monoclonal antibody to NEK11 on formalin-fixed paraffin-embedded human endometrium tissue. [antibody concentration 3ug/ml].
Mouse Anti-NEK11 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS647920

Immunofluorescence (IF) of anti-NEK11 antibody

Immunofluorescence of monoclonal antibody to NEK11 on HeLa cell. [antibody concentration 10ug/ml].
Mouse Anti-NEK11 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS647920

Testing Data of anti-NEK11 antibody

Detection limit for recombinant GST tagged NEK11 is 0.1ng/ml as a capture antibody.
Mouse Anti-NEK11 Monoclonal Antibody, Testing Data – Cat# MBS647920

Western Blot (WB) of anti-NEK11 antibody

NEK11 monoclonal antibody, Western Blot analysis of NEK11 expression in HL-60.
Mouse Anti-NEK11 Monoclonal Antibody, Western Blot (WB) – Cat# MBS647920

Western Blot (WB) of anti-NEK11 antibody

NEK11 monoclonal antibody. Western Blot analysis of NEK11 expression in A-431.
Mouse Anti-NEK11 Monoclonal Antibody, Western Blot (WB) – Cat# MBS647920

Other Notes

Small volumes of anti-NEK11 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NEK11 purchase

MBS647920 is an antibody targeting NEK11. Also known as NEK11 (Serine/Threonine-protein Kinase Nek11, Never in Mitosis A-related Kinase 11, NimA-related Protein Kinase 11, FLJ23495). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NEK11. It may not necessarily be applicable to this product.

Protein Family
Serine/threonine-protein kinase

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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