• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Hepatoma Derived Growth Factor (HDGF) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6480049

MBS6480049 is an antibody targeting HDGF. Also known as Hepatoma Derived Growth Factor, CT (HDGF, DKFZp686J1764, FLJ96580, High-Mobility Group Protein 1-Like 2, HMG1L2, HMG-1L2) (FITC). Reactive in Human. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Hepatoma Derived Growth Factor (HDGF), Polyclonal Antibody
Full Product Name
Hepatoma Derived Growth Factor, CT (HDGF, DKFZp686J1764, FLJ96580, High-Mobility Group Protein 1-Like 2, HMG1L2, HMG-1L2) (FITC)
Product Gene Name
anti-HDGF antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human HDGF.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Molecular Weight
11.5 kDa (100aa), confirmed by MALDI-TOF.
OMIM
600339
3D Structure
ModBase 3D Structure for P51858
Immunogen
Synthetic peptide selected from the C-terminal region of human HDGF (KLH).
Conjugate
FITC
Note
Preservative Free

Product Categories/Family for anti-HDGF antibody

Antibodies; Growth Factors; Cytokines
Applications Tested/Suitable for anti-HDGF antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-HDGF antibody
IHC: 1:10-1:50
ELISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-HDGF antibody

Western blot analysis of anti-HDGF Pab in Hela cell line lysates (35ug/lane). HDGF(arrow) was detected using the purified Pab.
Rabbit Anti-HDGF Polyclonal Antibody – Cat# MBS6480049

Immunohistochemistry (IHC) of anti-HDGF antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HDGF antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-HDGF Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6480049

Immunofluorescence (IF) of anti-HDGF antibody

Confocal immunofluorescent analysis of HDGF Antibody (C-term) with SK-BR-3 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-HDGF Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6480049

Product References and Citations for anti-HDGF antibody

1. Thakar, K., FEBS J. 275(7), 1411-1426 (2008). 2. Chang, K.C., Int. J. Cancer 121(5), 1059-1065 (2007). 3. Yamamoto, S., Ann. Surg. Oncol. 14(7), 2141-2149 (2007). 4. Uyama, H., Clin. Cancer Res. 12(20 PT1), 6043-6048 (2006).

Other Notes

Small volumes of anti-HDGF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HDGF. It may not necessarily be applicable to this product.

NCBI GI #
4758516
NCBI GeneID
3068
NCBI Accession #
NP_004485
UniProt Primary Accession #
P51858
UniProt Secondary Accession #
Q5SZ07; Q5SZ08; Q5SZ09; B3KU21; D3DVC9
UniProt Related Accession #
P51858
GenBank Nucleotide #
NM_004494.2
NCBI Official Full Name
hepatoma-derived growth factor isoform a
NCBI Official Synonym Full Names
heparin binding growth factor
NCBI Official Symbol
HDGF
NCBI Official Synonym Symbols
HMG1L2
UniProt Protein Name
Hepatoma-derived growth factor
UniProt Synonym Protein Names
High mobility group protein 1-like 2
Protein Family
Hepatoma-derived growth factor
UniProt Gene Name
HDGF
UniProt Synonym Gene Names
HMG1L2; HDGF; HMG-1L2
UniProt Entry Name
HDGF_HUMAN
NCBI Protein Information
hepatoma-derived growth factor

NCBI Summary for HDGF

This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2016]
Show more

UniProt Comments for HDGF

HDGF: Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor. Belongs to the HDGF family.

Protein type: Transcription regulation; Cytokine

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: nucleoplasm; extracellular space; transcriptional repressor complex; cytoplasm

Molecular Function: heparin binding; DNA binding; growth factor activity; nucleotide binding

Biological Process: cell proliferation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; transcription, DNA-dependent; unfolded protein response; negative regulation of transcription from RNA polymerase II promoter; signal transduction
Show more

Research Articles on HDGF

1. Data suggested that HDGF knockdown inhibits cellular migration and invasion in vitro of prostate cancer via modulating epithelial-mesenchymal transition signaling pathway, as well as MMP2 and MMP9 signaling pathway. These results supported that HDGF is a relevant protein in the progression of prostate cancer and may serve as a potentially therapeutic target for prostate cancer as well as its downstream targets.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HDGF antibody

 Reference Product  PubMed Publications
 SMYD1 antibody  >3 publications with HDGF and SMYD1
 HMGB1 antibody  >2 publications with HDGF and HMGB1
 HMG1 antibody  >2 publications with HDGF and HMG1
 EEF2 antibody  >1 publications with HDGF and EEF2
 TP53 antibody  >1 publications with HDGF and TP53

Pathways associated with anti-HDGF antibody

 Products by Pathway  Pathway Diagram
 IRE1alpha Activates Chaperones Pathway antibodies  IRE1alpha Activates Chaperones Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Unfolded Protein Response (UPR) Pathway antibodies  Unfolded Protein Response (UPR) Pathway Diagram
 XBP1(S) Activates Chaperone Genes Pathway antibodies  XBP1(S) Activates Chaperone Genes Pathway Diagram

Diseases associated with anti-HDGF antibody

 Disease Name  Pubmed Publications
 Carcinoma, Hepatocellular Antibodies  >16 publications with HDGF and Carcinoma, Hepatocellular
 Liver Diseases Antibodies  >16 publications with HDGF and Liver Diseases
 Liver Neoplasms Antibodies  >16 publications with HDGF and Liver Neoplasms
 Gastrointestinal Diseases Antibodies  >12 publications with HDGF and Gastrointestinal Diseases
 Lung Diseases Antibodies  >11 publications with HDGF and Lung Diseases
 Lung Neoplasms Antibodies  >11 publications with HDGF and Lung Neoplasms
 Necrosis Antibodies  >6 publications with HDGF and Necrosis
 Cell Transformation, Neoplastic Antibodies  >4 publications with HDGF and Cell Transformation, Neoplastic
 Neoplasms, Experimental Antibodies  >3 publications with HDGF and Neoplasms, Experimental
 Pancreatic Neoplasms Antibodies  >3 publications with HDGF and Pancreatic Neoplasms

Organs/Tissues associated with anti-HDGF antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >23 publications with HDGF and Liver
 Vascular Antibodies  >22 publications with HDGF and Vascular
 Lung Antibodies  >22 publications with HDGF and Lung
 Blood Antibodies  >14 publications with HDGF and Blood
 Muscle Antibodies  >13 publications with HDGF and Muscle
 Brain Antibodies  >11 publications with HDGF and Brain
 Kidney Antibodies  >7 publications with HDGF and Kidney
 Embryonic Tissue Antibodies  >6 publications with HDGF and Embryonic Tissue
 Testis Antibodies  >6 publications with HDGF and Testis
 Nerve Antibodies  >5 publications with HDGF and Nerve
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000