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Hexokinase, Type IV (HKIV) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6480271

MBS6480271 is an antibody targeting HKIV. Also known as Hexokinase, Type IV, NT (HK IV, HKIV, Hexokinase-4, HK4, FGQTL3, Glucokinase, GCK, GK, GLK, Hexokinase-D, HHF3, HXKP, LGLK, MODY2) (PE). Reactive in Human, Mouse. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Hexokinase, Type IV (HKIV), Polyclonal Antibody
Full Product Name
Hexokinase, Type IV, NT (HK IV, HKIV, Hexokinase-4, HK4, FGQTL3, Glucokinase, GCK, GK, GLK, Hexokinase-D, HHF3, HXKP, LGLK, MODY2) (PE)
Product Gene Name
Product Synonym Names
EC=2.7.1.2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein G Affinity Chromatography.
Specificity
Recognizes human GCK. Species Crossreactivity: mouse.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
465
OMIM
phenotype 606391
3D Structure
ModBase 3D Structure for P35557
Immunogen
Synthetic peptide selected from the N-terminal region of human GCK (KLH).
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-HKIV antibody

Applications Tested/Suitable for anti-HKIV antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-HKIV antibody
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.

Immunofluorescence (IF) of anti-HKIV antibody

Confocal immunofluorescent analysis of GCK Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-HKIV Polyclonal Antibody – Cat# MBS6480271

Western Blot (WB) of anti-HKIV antibody

The anti-GCK Pab is used in Western blot to detect GCK in HepG2 cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2).
Rabbit Anti-HKIV Polyclonal Antibody, Western Blot (WB) – Cat# MBS6480271

Immunohistochemistry (IHC) of anti-HKIV antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-HKIV Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6480271

Product References and Citations for anti-HKIV antibody

1. Gloyn, A.L., et al., Diabetes 52(9):2433-2440 (2003). 2. Pruhova, S., et al., Diabetologia 46(2):291-295 (2003). 3. Rizzo, M.A., et al., J. Biol. Chem. 277(37):34168-34175 (2002). 4. Cao, H., et al., Hum. Mutat. 20(6):478-479 (2002). 5. Barrio, R., et al., J. Clin. Endocrinol. Metab. 87(6):2532-2539 (2002).

Other Notes

Small volumes of anti-HKIV antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HKIV. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Glucokinase
NCBI Official Synonym Full Names
glucokinase (hexokinase 4)
NCBI Official Symbol
GCK
NCBI Official Synonym Symbols
GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3
UniProt Protein Name
Glucokinase
UniProt Synonym Protein Names
Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D
UniProt Gene Name
GCK
UniProt Synonym Gene Names
HK IV; HK4
UniProt Entry Name
HXK4_HUMAN
NCBI Protein Information
glucokinase; HK IV; hexokinase-4; hexokinase-D; hexokinase type IV; hexokinase D, pancreatic isozyme; ATP:D-hexose 6-phosphotransferase

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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