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med20 Antibody – Mouse Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648045

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase… More details

$880 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MED20, Polyclonal Antibody
Full Product Name
MED20 (Mediator of RNA Polymerase II Transcription Subunit 20, Mediator Complex Subunit 20, TRF-proximal Protein Homolog, hTRFP, TRFP)
Product Gene Name
Product Synonym Names
Anti -MED20 (Mediator of RNA Polymerase II Transcription Subunit 20, Mediator Complex Subunit 20, TRF-proximal Protein Homolog, hTRFP, TRFP)
Immunogen Sequence
MGVTCVSQMP VAEGKSVQQT VELLTRKLEM LGAEKQGTFC VDCETYHTAA STLGSQGQTG KLMYVMHNSE YPLSCFALFE NGPCLIADTN FDVLMVKLKG FFQSAKASKI ETRGTRYQYC DFLVKVGTVT MGPSARGISV EVEYGPCVVA SDCWSLLLEF LQSFLGSHTP GAPAVFGNRH DAVYGPADTM VQYMELFNKI RKQQQVPVAG IR
Clonality
Polyclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human MED20.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
22,350 Da
Chromosome Location
Chromosome: I; NC_003424.3 (2350014..2351244). Location: chromosome: I
3D Structure
ModBase 3D Structure for Q10317
Immunogen
Full length human MED20, aa1-212 (NP_004266.2).
Applications Tested/Suitable for anti-med20 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-med20 antibody
Suitable for use in Immunofluorescence and Western Blot.
Dilution: Immunofluorescence: 10ug/ml

Western Blot (WB) of anti-med20 antibody

TRFP polyclonal antibody. Western Blot analysis of TRFP expression in Hela NE.
Mouse Anti-med20 Polyclonal Antibody – Cat# MBS648045

Western Blot (WB) of anti-med20 antibody

Western Blot analysis of MED20 expression in transfected 293T cell line by MED20 polyclonal antibody. Lane 1: TRFP transfected lysate (23.32kD). Lane 2: Non-transfected lysate.
Mouse Anti-med20 Polyclonal Antibody, Western Blot (WB) – Cat# MBS648045

Immunofluorescence (IF) of anti-med20 antibody

Immunofluorescence of purified antibody to TRFP on HeLa cell. [antibody concentration 10ug/ml]
Mouse Anti-med20 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS648045

Other Notes

Small volumes of anti-med20 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for med20 purchase

MBS648045 is an antibody targeting med20. Also known as MED20 (Mediator of RNA Polymerase II Transcription Subunit 20, Mediator Complex Subunit 20, TRF-proximal Protein Homolog, hTRFP, TRFP). Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for med20. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
mediator complex subunit Med20
NCBI Official Symbol
med20
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 20
UniProt Synonym Protein Names
Mediator complex subunit 20
UniProt Gene Name
med20
UniProt Entry Name
MED20_SCHPO
NCBI Protein Information
mediator complex subunit Med20

UniProt Comments for med20

Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Ref.4

Subunit structure: Component of the Mediator complex. Ref.4

Subcellular location: Nucleus Ref.3.

Sequence similarities: Belongs to the Mediator complex subunit 20 family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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