• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Histone H3 (HIST3H3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6480684

MBS6480684 is an antibody targeting HIST3H3. Also known as Histone H3, acetylated (Lys9, Lys14) (MaxLight 550). Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$1525 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Histone H3 (HIST3H3), Polyclonal Antibody
Full Product Name
Histone H3, acetylated (Lys9, Lys14) (MaxLight 550)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human acetylated histone H3 at the N-terminal at ~17kD. Species Crossreactivity: mouse and rat.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
136
Chromosome Location
Chromosome: 1; NC_000001.10 (228612546..228613026, complement). Location: 1q42
OMIM
602820
3D Structure
ModBase 3D Structure for Q16695
Immunogen
Synthetic peptide corresponding to aa1-20 of Tetrahymena histone H3 (ARTKQTAR[K*]STGG[K*]APRKQLC: where K* is acetylated) (KLH).
Conjugate
MaxLight550
Antigen Modification
Acetylated
Note
Preservative Free

Product Categories/Family for anti-HIST3H3 antibody

Applications Tested/Suitable for anti-HIST3H3 antibody
Immunocytochemistry (ICC), Western Blot (WB), Chromatin Immunoprecipitation (ChIP)
Application Notes for anti-HIST3H3 antibody
ICC: 1:500 detected acetylated Histone H3 in NIH/3T3 and A431 cells.
WB: 0.05ug/ml detected Histone H3 on 10ug of HeLa acid extract, sodium butyrate treated HeLa cells and recombinant Histone H3. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemilumnescence detection system.
Applications are based on unconjugated antibody.

Testing Data of anti-HIST3H3 antibody

HeLa acid extract (lane1), Sodium butyrate treated HeLa cells (lane 2) and recombinant Histone H3 (Lane3) lysates were probed with MBS617139 (0.05ug/ml). Proteins were visualized using a anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system. Arrow indicates Histone H3 (~17kD).
Rabbit Anti-HIST3H3 Polyclonal Antibody – Cat# MBS6480684

Testing Data of anti-HIST3H3 antibody

Confocal fluorescent analysis of A431 and NIH/3T3 cells using MBS617139 (Red). Actin filaments have been labeled with AlexaFluor488-Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Rabbit Anti-HIST3H3 Polyclonal Antibody, Testing Data – Cat# MBS6480684

Product References and Citations for anti-HIST3H3 antibody

1. Kashiwaya K, et al. (2010). Cancer Res. 70:4024-4033. 2. Dispirito JR, and Shen H. (2010). J. Immunol. 184:4631-4636. 3. Turnbaugh PJ, et al. (2010). Proc. Natl. Acad Sci. USA. 107:7503-7508. 4. Evans P.M., et al. (2009). Mol. Cell. Bio. 30:372-381. 5. Zippo, A., et al. (2009). Cell. 138: 1122-1136. 6. Dammer, EB., et al. (2007). Mol Endocrinol. 21: 415-38. 7. Liu, Chang, et al. (2007). Nature. 447: 477-81. 8. Marban, Celine, et al. (2007). EMBO J. 26:412-23. 9. Benhamed, Moussa, et al. (2006). Plant Cell. 18: 2893-903. 10. Guyot, B., et al. (2004). Blood. 104: 89-91. 11. Detich, N., et al. (2003). J. Biol. Chem. 278:27586-27592. 12. Rastegar, M., et al. (2004). Mol Cell Biol. 24:8090-103.

Other Notes

Small volumes of anti-HIST3H3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HIST3H3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
histone H3.1t
NCBI Official Synonym Full Names
histone cluster 3, H3
NCBI Official Symbol
HIST3H3
NCBI Official Synonym Symbols
H3t; H3.4; H3/g; H3FT
UniProt Protein Name
Histone H3.1t
UniProt Synonym Protein Names
H3/g
Protein Family
UniProt Gene Name
HIST3H3
UniProt Synonym Gene Names
H3FT; H3/t; H3t
UniProt Entry Name
H31T_HUMAN
NCBI Protein Information
histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000