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NUDT21 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648072

CPSF5 is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitement… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
NUDT21, Monoclonal Antibody
Full Product Name
NUDT21 (Cleavage and Polyadenylation Specificity Factor Subunit 5, Cleavage and Polyadenylation Specificity Factor 25kD Subunit, CPSF 25kD Subunit, Pre-mRNA Cleavage Factor Im 25kD Subunit, Nucleoside Diphosphate-linked Moiety X Motif 21, Nudix Motif 21
Product Gene Name
Product Synonym Names
Anti -NUDT21 (Cleavage and Polyadenylation Specificity Factor Subunit 5, Cleavage and Polyadenylation Specificity Factor 25kD Subunit, CPSF 25kD Subunit, Pre-mRNA Cleavage Factor Im 25kD Subunit, Nucleoside Diphosphate-linked Moiety X Motif 21, Nudix Motif 21
Immunogen Sequence
MSVVPPNRSQ TGWPRGVTQF GNKYIQQTKP LTLERTINLY PLTNYTFGTK EPLYEKDSSV AARFQRMREE FDKIGMRRTV EGVLIVHEHR LPHVLLLQLG TTFFKLPGGE LNPGEDEVEG LKRLMTEILG RQDGVLQDWV IDDCIGNWWR PNFEPPQYPY IPAHITKPKE HKKLFLVQLQ EKALFAVPKN YKLVAAPLFE LYDNAPGYGP IISSLPQLLS RFNFIYN
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3F8
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human NUDT21.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
22,636 Da
Chromosome Location
Chromosome: 1; NC_003070.9 (27645658..27646698, complement). Location: chromosome: 1
3D Structure
ModBase 3D Structure for Q8VY81
Immunogen
Full length recombinant corresponding to aa1-228 from human NUDT21 (AAH01403) with GST tag. MW of the GST tag alone is 26kD.
Applications Tested/Suitable for anti-NUDT21 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-NUDT21 antibody
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Dilution: Immunofluorescence: 10ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml

Western Blot (WB) of anti-NUDT21 antibody

Western Blot detection against Immunogen (51.08kD).
Mouse Anti-NUDT21 Monoclonal Antibody – Cat# MBS648072

Immunohistochemistry (IHC) of anti-NUDT21 antibody

Immunoperoxidase of monoclonal antibody to NUDT21 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].
Mouse Anti-NUDT21 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS648072

Immunofluorescence (IF) of anti-NUDT21 antibody

Immunofluorescence of monoclonal antibody to NUDT21 on HeLa cell . [antibody concentration 10ug/ml].
Mouse Anti-NUDT21 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS648072

Testing Data of anti-NUDT21 antibody

Detection limit for recombinant GST tagged NUDT21 is ~3ng/ml as a capture antibody.
Mouse Anti-NUDT21 Monoclonal Antibody, Testing Data – Cat# MBS648072

Other Notes

Small volumes of anti-NUDT21 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NUDT21 purchase

MBS648072 is an antibody targeting NUDT21. Also known as NUDT21 (Cleavage and Polyadenylation Specificity Factor Subunit 5, Cleavage and Polyadenylation Specificity Factor 25kD Subunit, CPSF 25kD Subunit, Pre-mRNA Cleavage Factor Im 25kD Subunit, Nucleoside Diphosphate-linked Moiety X Motif 21, Nudix Motif 21. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NUDT21. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nudix hydrolase 21
NCBI Official Symbol
NUDT21
NCBI Official Synonym Symbols
atnudt21; nudix hydrolase homolog 21; NUDT21; T9L24.30; T9L24_30
UniProt Protein Name
Nudix hydrolase 21, chloroplastic
Protein Family
UniProt Gene Name
NUDT21
UniProt Synonym Gene Names
NUDX21; AtNUDT21
UniProt Entry Name
NUD21_ARATH
NCBI Protein Information
nudix hydrolase 21

UniProt Comments for NUDT21

Function: Probably mediates the hydrolysis of some nucleoside diphosphate derivatives

By similarity.

Cofactor: Magnesium or manganese

By similarity.

Subcellular location: Plastid › chloroplast

Potential.

Tissue specificity: Expressed in roots, leaves, stems and inflorescences. Ref.5

Disruption phenotype: No visible phenotype under normal growth conditions. Ref.5

Sequence similarities: Belongs to the Nudix hydrolase family.Contains 1 nudix hydrolase domain.

Sequence caution: The sequence AAG30977.1 differs from that shown. Reason: Erroneous initiation.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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