Rabbit Anti-PAPOLG Polyclonal Antibody – Cat# MBS648087

PAPOLG Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PAPOLG antibody detected the PAPOLG protein (arrow).

PAPOLG Antibody – Rabbit Polyclonal

Name: PAPOLG Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS648087
Price: 895 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648087

This gene encodes a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level.… More details

$895 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PAPOLG, Polyclonal Antibody

Full Product Name

PAPOLG, CT (PAPOLG, PAP2, PAPG, Poly(A) polymerase gamma, Neo-poly(A) polymerase, Polynucleotide adenylyltransferase gamma, SRP RNA 3'-adenylating enzyme)

Product Gene Name

anti-PAPOLG antibody

Product Synonym Names

Anti -PAPOLG, CT (PAPOLG, PAP2, PAPG, Poly(A) polymerase gamma, Neo-poly(A) polymerase, Polynucleotide adenylyltransferase gamma, SRP RNA 3'-adenylating enzyme)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Human

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

82,803 Da

Chromosome Location

Chromosome: 2; NC_000002.11 (60983365..61029221). Location: 2p16.1

3D Structure

ModBase 3D Structure for Q9BWT3

Immunogen

PAPOLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 684-714 amino acids from the C-terminal region of human PAPOLG.

Product Categories/Family for anti-PAPOLG antibody

Antibodies; Abs to Enzymes; Polymerase

Applications Tested/Suitable for anti-PAPOLG antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-PAPOLG antibody

Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Other Notes

Small volumes of anti-PAPOLG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAPOLG purchase

MBS648087 is an antibody targeting PAPOLG. Also known as PAPOLG, CT (PAPOLG, PAP2, PAPG, Poly(A) polymerase gamma, Neo-poly(A) polymerase, Polynucleotide adenylyltransferase gamma, SRP RNA 3'-adenylating enzyme). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PAPOLG. It may not necessarily be applicable to this product.

NCBI GI #

16306568

NCBI GeneID

64895

NCBI Accession #

NP_075045.2

UniProt Primary Accession #

Q9BWT3

UniProt Secondary Accession #

Q969N1; Q9H8L2; Q9HAD0; B2RBH4

UniProt Related Accession #

Q9BWT3

GenBank Nucleotide #

NM_022894.3

NCBI Official Full Name

poly(A) polymerase gamma

NCBI Official Synonym Full Names

poly(A) polymerase gamma

NCBI Official Symbol

PAPOLG

UniProt Protein Name

Poly(A) polymerase gamma

UniProt Synonym Protein Names

Neo-poly(A) polymerase; Neo-PAP; Polynucleotide adenylyltransferase gamma; SRP RNA 3'-adenylating enzyme

Protein Family

Poly(A) polymerase

UniProt Gene Name

PAPOLG

UniProt Synonym Gene Names

PAP2; PAPG; PAP-gamma; Neo-PAP

UniProt Entry Name

PAPOG_HUMAN

NCBI Protein Information

poly(A) polymerase gamma; poly(A) polymerase gamma; neo-PAP; PAP-gamma; neo-poly(A) polymerase; SRP RNA 3'-adenylating enzyme; nuclear poly(A) polymerase gamma; SRP RNA 3' adenylating enzyme/pap2; polynucleotide adenylyltransferase gamma

UniProt Comments for PAPOLG

Function: Responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.

Catalytic activity: ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactor: Binds 2 magnesium ions. Also active with manganese

By similarity.

Subcellular location: Nucleus Ref.2.

Tissue specificity: Expressed predominantly in testis, and weakly in other tissues. Overexpressed in several tumors. Ref.2

Sequence similarities: Belongs to the poly(A) polymerase family.

Sequence caution: The sequence BAB14604.1 differs from that shown. Reason: Erroneous initiation.

Research Articles on PAPOLG

1. PAP gamma structurally closely resembles its PAP alpha ortholog. It possesses a conserved catalytic binding pocket, whereas residues at the surface of the polymerase are more divergent.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PAPOLG antibody

Products by Pathway	Pathway Diagram
MRNA Surveillance Pathway antibodies	MRNA Surveillance Pathway Diagram
MRNA Surveillance Pathway antibodies	MRNA Surveillance Pathway Diagram

Diseases associated with anti-PAPOLG antibody

Disease Name	Pubmed Publications
Aneuploidy Antibodies	>1 publications with PAPOLG and Aneuploidy

Organs/Tissues associated with anti-PAPOLG antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>1 publications with PAPOLG and Brain