DNAJA3 Antibody – Rabbit Polyclonal

This gene encodes a member of the DNAJ/Hsp40 protein family. DNAJ/Hsp40 proteins stimulate the ATPase activity of Hsp70 chaperones and play critical roles in protein folding, degradation, and multimeric complex assembly. The encoded protein is localized to mitochondria and mediates several cellular processes including proliferation, survival and apoptotic signal transduction. The encoded protein also plays a critical role in tumor suppression through interactions with oncogenic proteins including ErbB2 and the p53 tumor suppressor protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]

Function: Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway.

Subunit structure: Interacts with JAK2, HSPA9B and IFN-gammaR2 chain. Interacts with Ras GTPase-activating protein 1 (RASA1). Isoform 2 interacts with MUSK (via the cytoplasmic domain)

By similarity. Ref.2

Subcellular location: Mitochondrion matrix. Cytoplasm › cytosol

By similarity. Cell junction › synapse › postsynaptic cell membrane; Peripheral membrane protein

By similarity. Note: Recruited to the postsynaptic cell membrane of the neuromuscular junction through interaction with MUSK

By similarity.

Tissue specificity: Widely expressed with highest levels in heart, liver, lung and skeletal muscles. Also expressed in keratinocytes. Ref.1

Domain: Modulation of apoptosis, i.e. proapoptotic activity of isoform 1 and antiapoptotic activity of isoform 2, is J domain-dependent.

Post-translational modification: Tyrosine phosphorylated

By similarity.

Sequence similarities: Contains 1 CR-type zinc finger.Contains 1 J domain.

Sequence caution: The sequence AAF66245.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAF66245.1 differs from that shown. Reason: Frameshift at positions 408 and 411. The sequence AAH12343.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.