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TRIM54 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648212

The protein encoded by this gene contains a RING finger motif and is highly similar to the ring finger proteins RNF28/MURF1 and RNF29/MURF2. In vitro studies demonstrated that this protein, RNF28, and RNF29 form heterodimers, which may be important for the… More details

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Sample Manual Insert View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
TRIM54, Polyclonal Antibody
Full Product Name
TRIM54, NT (TRIM54, MURF, MURF3, RNF30, Tripartite motif-containing protein 54, Muscle-specific RING finger protein, Muscle-specific RING finger protein 3, RING finger protein 30)
Product Gene Name
Product Synonym Names
Anti -TRIM54, NT (TRIM54, MURF, MURF3, RNF30, Tripartite motif-containing protein 54, Muscle-specific RING finger protein, Muscle-specific RING finger protein 3, RING finger protein 30)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
41,337 Da
Chromosome Location
Chromosome: 11; AC_000168.1 (72327350..72349010, complement). Location: chromosome: 11
3D Structure
ModBase 3D Structure for Q58D15
Immunogen
TRIM54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TRIM54.
Applications Tested/Suitable for anti-TRIM54 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-TRIM54 antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50

Western Blot (WB) of anti-TRIM54 antibody

TRIM54 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the TRIM54 antibody detected the TRIM54 protein (arrow).
Rabbit Anti-TRIM54 Polyclonal Antibody – Cat# MBS648212

Immunohistochemistry (IHC) of anti-TRIM54 antibody

TRIM54 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM54 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-TRIM54 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS648212

Other Notes

Small volumes of anti-TRIM54 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRIM54 purchase

MBS648212 is an antibody targeting TRIM54. Also known as TRIM54, NT (TRIM54, MURF, MURF3, RNF30, Tripartite motif-containing protein 54, Muscle-specific RING finger protein, Muscle-specific RING finger protein 3, RING finger protein 30). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TRIM54. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tripartite motif-containing protein 54
NCBI Official Synonym Full Names
tripartite motif containing 54
NCBI Official Symbol
TRIM54
UniProt Protein Name
Tripartite motif-containing protein 54
UniProt Gene Name
TRIM54
UniProt Entry Name
TRI54_BOVIN
NCBI Protein Information
tripartite motif-containing protein 54; ring finger protein 30; tripartite motif-containing 54

UniProt Comments for TRIM54

Function: May bind and stabilize microtubules during myotubes formation

By similarity.

Subunit structure: Homooligomer and heterooligomer. Interacts with TRIM63 and probably with TRIM55. Interacts with tubulin

By similarity.

Subcellular location: Cytoplasm › cytoskeleton

By similarity. Cytoplasm › myofibril › sarcomere › Z line

By similarity. Note: Associates with microtubules. Localizes to the Z-lines in skeletal muscles

By similarity.

Sequence similarities: Contains 1 B box-type zinc finger.Contains 1 COS domain.Contains 1 RING-type zinc finger.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-TRIM54 antibody

 Disease Name  Pubmed Publications
 Atrophy Antibodies  >3 publications with TRIM54 and Atrophy

Organs/Tissues associated with anti-TRIM54 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >6 publications with TRIM54 and Muscle
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