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C12orf53 (PIANP) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648252

MBS648252 is an antibody targeting PIANP. Also known as C12orf53, NT (C12orf53, PILR-associating neural protein, Paired immunoglobin-like type 2 receptor-associating neural protein). Reactive in Human, Mouse. Host: Rabbit. The function of this protein has not… More details

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Product Overview

Online Datasheet in PDF
Product Name
C12orf53 (PIANP), Polyclonal Antibody
Full Product Name
C12orf53, NT (C12orf53, PILR-associating neural protein, Paired immunoglobin-like type 2 receptor-associating neural protein)
Product Gene Name
Product Synonym Names
Anti -C12orf53, NT (C12orf53, PILR-associating neural protein, Paired immunoglobin-like type 2 receptor-associating neural protein)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human, mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
30,076 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (6802957..6810009, complement). Location: 12p13.31
3D Structure
ModBase 3D Structure for Q8IYJ0
Immunogen
C12orf53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-117 amino acids from the N-terminal region of human C12orf53.

Product Categories/Family for anti-PIANP antibody

Applications Tested/Suitable for anti-PIANP antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-PIANP antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-PIANP antibody

Western blot analysis of C12orf53 Antibody (N-term) in mouse liver tissue lysates (35ug/lane).C12orf53 (arrow) was detected using the purified Pab.
Rabbit Anti-PIANP Polyclonal Antibody – Cat# MBS648252

Immunohistochemistry (IHC) of anti-PIANP antibody

C12orf53 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C12orf53 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-PIANP Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS648252

Flow Cytometry (FC/FACS) of anti-PIANP antibody

C12orf53 Antibody (N-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-PIANP Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS648252

Other Notes

Small volumes of anti-PIANP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PIANP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
chromosome 12 open reading frame 53, isoform CRA_d
NCBI Official Synonym Full Names
PILR alpha associated neural protein
NCBI Official Symbol
PIANP
NCBI Official Synonym Symbols
PANP; leda-1; C12orf53
UniProt Protein Name
PILR alpha-associated neural protein
UniProt Synonym Protein Names
PILR-associating neural protein; Paired immunoglobin-like type 2 receptor-associating neural protein
UniProt Gene Name
PIANP
UniProt Synonym Gene Names
C12orf53; PANP
UniProt Entry Name
PIANP_HUMAN
NCBI Protein Information
PILR alpha-associated neural protein; PILR-associating neural protein; liver endothelial differentiation-associated protein-1; paired immunoglobin-like type 2 receptor-associating neural protein

NCBI Summary for PIANP

This gene encodes a ligand for the paired immunoglobin-like type 2 receptor alpha, and so may be involved in immune regulation. Alternate splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Sep 2011]

UniProt Comments for PIANP

Function: Acts as a ligand for PILRA in neural tissues, where it may be involved in immune regulation. Ref.1

Subcellular location: Membrane; Single-pass type I membrane protein

Potential.

Tissue specificity: Mainly expressed in adult brain and cerebellum. Weaker expression in fetal brain and virtually no expression in spleen, heart, kidney, liver and dorsal ganglion relative to brain. Ref.1

Post-translational modification: O-glycosylation at Thr-140 is essential for recognition by PILRA

By similarity.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-PIANP antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1 publications with PIANP and Brain
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